Multi-Photon Laser Scanning Microscope with FLIM

所要求的显微镜系统将被整合到现有的核心设施“双光子成像”中，这是亚琛RWTH大学医学院内亚琛跨学科临床研究中心的核心设施。如补充研究中所述，显微镜系统将用于各种项目，以阐明不同研究领域的开放问题，包括：固体器官纤维化(肾脏、心脏、肺)和血管损伤与修复，包括动脉和动脉硬化；炎症过程的分子机制，特别是ADAM(EtallProtease)S；细胞骨架作为细胞和组织机械性能的主要提供者；STAT3相关疾病模型中细胞因子信号传递的分子机制；急性呼吸窘迫综合征的病理生理学；慢性肾脏疾病中与导致疾病发展和进展的三维细胞形态变化相关的病理生理变化；慢性肾脏疾病进展过程中肾脏纤维化和心血管疾病发病的病理生理学机制；以及心血管疾病和慢性肾脏疾病背景下的免疫-脂质串扰。

The requested microscope system will be integrated into the existing Core Facility “Two-Photon Imaging”, a Core Facility of the Interdisciplinary Centre for Clinical Research (IZKF) Aachen within the Faculty of Medicine at RWTH Aachen University. As described in the supplement research the microscope system will be used in various projects to elucidate open questions in different fields of research including: solid organ fibrosis (kidney, heart, lung) and vascular injury and repair, including athero- and arteriosclerosis; molecular mechanisms of inflammatory processes with a special focus on ADAM(etalloprotease)s; cytoskeleton function as the main provider of mechanical properties of cells and tissues; molecular mechanisms of cytokine signalling in STAT3-relevant disease models, pathophysiology of acute respiratory distress syndrome; pathophysiological changes in chronic kidney diseases associated with changes in the three-dimensional cellular morphology that lead to disease development and progression; pathophysiological mechanisms of renal fibrosis and the onset of cardiovascular disease developed during progressive chronic kidney disease; and immune-lipid crosstalk in the setting of both cardiovascular disease and chronic kidney disease.