Study of the Polymer Compounds on the Stability of Enzyme Activity and its Builder Actions

从含酶洗涤剂节能、环保和高性能的角度出发，研究了助建剂结构对酶活性稳定性及其助建剂作用的影响。阴离子表面活性剂、dbs和某些电解质对蛋白酶和脂肪酶活性的影响与Ca^<2+>的结合能力成正比。另一方面，尽管在含有0.02% DBS和/或0.02%电解质助凝剂的溶液中，添加非电解质PVAs和Ca^<2+>可激活蛋白酶。结果表明，除明胶土壤外，明胶和氧化铁污染棉织物的颗粒氧化铁也会对溶液中蛋白酶的活性产生很大的影响。脂肪酶的活性导致其在碱性溶液中的稳定性较低，但在洗涤后的织物上确实发现了三油酸土壤的水解产物。这些结果表明，以酶的稳定性能优于酶的诱骗性能为标准，构建离子单体/非离子单体的共聚物是优选的。结果表明：2-羟乙基甲基丙烯酸酯；苯乙烯磺酸钠；甲基丙烯酸钠共聚物具有与酶的低相互作用和Ca^<2+>结合能力等助聚剂作用，是一种优良的助聚剂。

From the view points of energy-saving, environmental protection and high performance of enzyme-containing detergents, the effects of the construction of the builders on the stability of enzyme activity and its builder actions were studied. The activities of enzymes, protease or lipase, were lowered by anionic surfactant, dbs, and some electrolytes in proportion to the property of Ca^<2+> binding capactiy. The other, the protesase was activated by addition of nonelectrolyte PVAs and Ca^<2+>in spite of in the solution containing with 0.02% DBS and/or 0.02% electrolyte builder.It was resulted that the removal of soils should be much influenced by the activity of protease in the solution not only the soil of gelatin but also the particulate ferric oxide from cotton fabric soiled with gelatin and ferric oxide.The activity of lipase resulted in low stability in the alkaline solution,but the hydrolysates of triolein soil were certainly found on the washed fabric. These results suppose that the copolymers constructed ionicmonomer/nonionicmonomer should be preferred with the criterion rather the stabilization property of enzyme than the chetate property.Is was confirmed that the 2-hydroxyethylmethacrylate ; sodium styrene sulfonate ; sodium methacrylate copolymer is a excellent builder having the properities of low interaction with the enzymes and having the builder actions, such as Ca^<2+>binding capacity electrokinetic effect by the adsorption and suspension stability.