Study on Humoral Factor in Renal Allotransplantation with Special Regard to MLC Activation Factor

促使我们开展这一研究项目的是，我们注意到在我所接受同种异体肾移植的患者中，有4例患者术前血清中存在MLC激活因子(S)，并可能由于这些激活因子(S)的存在而经历了特殊的术后过程。根据对其临床病程的观察，认为这种MLC激活因子(S)可能诱发难治性排斥反应，这种作用可被DST所抑制。本研究旨在阐明MLC激活因子(S)的免疫机制。第一年：(1)经血清学试验证实激活因子(S)对MLC具有特异性。(2)将自回归模型作为急性排斥反应的诊断工具之一，对免疫网络进行分析。(3)观察门静脉给药对狗肾移植移植物存活时间的影响。第二年：(1)大鼠异基因输注后，测定腹腔巨噬细胞的前列腺素E和丙二醛的生成。静脉或门静脉注射DST的不同途径对移植物存活无明显影响，但其作用机制因输血途径不同而不同。(2)对急性排斥反应的免疫学诊断，实质上是观察移植肾这一靶器官发生的免疫现象。为此，有必要在安全和重复的基础上从肾脏获取试验标本。为了满足这一要求，采用杂种犬模型对细针吸取细胞学(FNAC)的可行性进行了研究。最后一年：尽一切努力做出最终报告，以便在医学大会上展示我们的结果并发表在医学期刊上。

What prompted us to conduct this research project was that we noted 4 particular cases among patients having undergone renal allotransplantion in our institution, who had MLC activation factor (s) in their serum prior to the operation and experienced peculiar post-operative courses presumably because of presence of these activation factor (s). Based on the observation of their clinical courses, it was considered that this MLC activation factor (s) might induce refractory rejection and this action could be suppressed by DST. The purpose of this study is to clarify the immune mechanism of the MLC activation factor (s).The 1st year : (1) specificity of the activation factor (s) was proved by serological test for MLC. (2) Immunological net work was analyzed by using self regression model as one of diagnostic tools for acute rejection. (3) The effect of DST via portal vein was examined on prolongation of graft survival in renal allotransplantation using mongrel dogs.The second year : (1) PGE & TBX productivity by intraperitoneal macrophages was examined after alogeneic transfusion in rats. The different routes of DST, intravenously or portal venously, did not make any difference in immunosuppressive effect in terms of graft survival but their mechanism seemed to be different according to the routes of transfusion. (2) In order to make immunological diagnosis of acute rejection, substantial way is to investigate the immunological phenomena taking place in the target organ, namely the transplanted kidney. For this purpose, it is neccessary to obtain the test specimens from the kidney on the safe and repeated basis. To meet this requirement, feasibility of fine needle aspiration cytology (FNAC) was examined by using mongrel dog model.The final year : All efforts were paid to make final report so that our results were presented at medical convention and also published in medical journals.