Phylogenetic Analysis of Coryneform and Related Bacteria Based on 5s rRNA Base Sequences

棒状细菌的一般概念主要基于其细胞壁的肽聚糖结构、甲基萘醌系统、细胞脂肪酸组成和DNA碱基组成等化学分类特征。为评价棒状细菌的分类系统，采用5s rRNA的碱基序列进行了遗传分析。根据Hori和Osawa的方法，将11株棒状菌及其相关生物的序列与Park等人之前的研究进行了比较。虽然Phodococcus、Nocardia和Mycobacterium属与棒状杆菌属具有相同的细胞壁结构，含有中二氨基戊二酸、阿拉伯半乳聚糖和霉菌酸，但前者属被列入Pard等人提出的棒状细菌含有非中二氨基戊二酸肽聚糖的亚群。棒状杆菌和短杆菌独立构建亚群。棒状细菌的鉴定菌株数量不足一直是棒状细菌分类学的难题。我们收集了未识别的棒状细菌并进行了化学分类。结果显示，其中包括很少分离的棒状细菌，如冢村菌和烟叶节杆菌群。这些菌株有望为棒状细菌分类的改进做出贡献。

The generic concept of coryneform bacteria ia mainly based on their chemotaxonomic characteristics such as peptidoglycan structure of cell wall, menaquinone systems, cellular fatty acid composition and DNA base composition, To evaluate the taxonomic system mentioned above, phyligenetic analysis with base sequence of 5s rRNA was carried ou. According to the method of Hori and Osawa, the sequences of eleven strains of coryneform and the related organisms are compared with the previous study by Park et al. Although the genera Phodococcus, Nocardia, and Mycobacterium share the same cell wall structucture containing meso-diaminopimelic acid, arabinogalactan, and mycolic acids with the genus Corynebacterium, the former genera were included in the subgroup proposed by Pard et al. which comprised coryneform bacteria with non-mesodiaminopimelic acid peptidoglycan. Corynebacterium and Brevibacterium constructed subgroups independently.It has been a problem of the taxonomy of coryneform bacteria that the authentic strains are not sufficient. We collected unidentified coryneform bacteria and chemotaxonomically characterized. It was revealed that they included rarely isolated coryneform bacteria such as Tsukamurera and the Arthrobacter nicotianae group. These strains are expected to contribute to the improvement of the taxonomy of coryneform bacteria.