Immunology and Molecular Biology of Cestode Infections.

用几种绦虫Hvmenolepis spp.分析了寄生虫感染过程中宿主与寄生虫的相互作用。和在小鼠和大鼠体内的带绦虫。至少需要两个不同的物种作为中间和最终宿主，才能完成绦虫的生活史，但不包括H.nana。在本工作中，我们分析了(1)成虫肠腔内成虫的免疫原性，重点分析了成熟成虫产生和释放的囊虫的命运；(2)分析了中间宿主组织中幼虫的免疫原性，重点分析了六钩虫的宿主保护性抗原。在前者中，(1)成虫具有免疫原性，但免疫原性不同于幼虫；(2)对幼虫或成虫的免疫是阶段特异性的，但并不总是物种特异性的。在绦虫的生活史上，我们发现了由成虫…产生和释放的六钩虫胚胎更多需要以甲虫为中间寄主的小口隐翅虫和小隐翅虫可能孵化并侵入最终寄主的肠道组织。虽然目前尚不清楚入侵最终宿主组织的六钩虫是否会发育成幼虫囊虫，但这一观察结果很好地解释了为什么感染微小毛滴虫的小鼠除了产生抗幼虫和抗六钩虫抗体外，还会产生抗六钩虫抗体，以及为什么给予这种寄生虫活卵的小鼠只产生抗六钩虫抗体，并对挑战感染六钩虫六钩虫球表现出很强的抵抗力。目前，我们可以推测在感染细粒棘球绦虫或具有医学意义的多房棘球绦虫的最终宿主犬中产生抗六钩虫抗体的原因。在后者中，我们已经证明了六钩虫球在哺乳动物中间宿主中作为免疫原的重要性。在与澳大利亚科学家的合作下，我们成功地建立了能产生21 kDa的六钩螺期特异性和宿主保护性多肽的大肠杆菌。这是通过重组DNA技术生产的第一个演示六钩螺期特异性成分。较少

The host-parasite interaction in cestode infections has been analyzed using several species of cestodes, Hvmenolepis spp. and Taenia taeniaeformis in mice and rats. At least two different species are required as the intermediate and definitive hosts for completion of the life-cycle of cestodes except H. nana. In the present work, we have analyzed (1) immunogenicity of adult cestodes in the intestinal lumen of deinitive host with emphasis on the fate of cncospheres produced by and released from mature adult worms, and (2) that of larval cestodes in the tissue of the intermediate host with emphasis on the host-protective antigens of the oncosphere. In the former, it has become clear that (1) adult cestodes are immunogenic but the immunogenicity differs from that of larval cestodes, (2) immunity to larval cestodes or adult cestodes is stage-specific but not always species-specific. On the life cycle of cestodes, we have found tht oncosphere embryos produced by and released from adult csto … More des of H. microstoma and H. diminuta, which require beetles as the intermediate host, may hatch and invade the intestinal tissue of the definitive host. Although it is open to question whether oncospheres invaded the tissue of the definitive host could develop into larval cstodes, this observation provides a good explanation as to why mice experienced patent infecion with H. microstoma produce anti-oncosphere antibodies in addition to anti-larval and anti-oncosphere antibodies and why mice given viable eggs of this parasite produce anti-oncosphere antibodies exclusively and show strong resistance to challenge infection with oncospheres of H. nana. At present, we may speculate the reason why anti-oncosphere antibodies are produced in the definitive host dogs infected with Echinocococcus granulosus or E, multilocularis of medical importance. In the latter, we have shown the importance of oncospheres as the immunogen in mammalian intermediate hosts. With collaboration with Australian scientists, we have succeeded in establishing Escherichia coli producing oncosphere stage-specific and host-protective polypeptide of 21 kDa. This is the first demonstrating oncosphere stage-specific component produced by recombinant DNA technology. Less