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Analysis of the sperm motility initiation substances in newt egg-jelly

蝾螈卵胶中精子活动启动物质的分析

基本信息

批准号：
12680710
负责人：
ONITAKE Kazuo
金额：
$ 2.24万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680710/
关键词：
internal fertilization amphibian egg-jelly sperm motility ion channel 受精卵ジェリー層両生類

项目摘要

Newt undergoes internal fertilization. Sperm are directly inseminated on the surface of egg-jelly in the cloaca of the female. We have reported that most sperm begin to move rapidly by the treatment of egg-jelly extract (JE) of the newt, cynops pyrrhogaster, and the sperm motility initiation factor (SMIS) in the JE is responsible for the rapid initiation of sperm motility. Egg-jelly also contains cations that can involve in the regulation of the sperm motility. In this study, we elucidated the role of those factors under the physiological condition of egg-jelly. The reconstructed ionic solution (RIS) was prepared according to the estimated pH and ionic concentrations. When dry sperm were added to the JE-RIS, most of them began to move within 3 min. A K^+-channel blocker, verapamil, and a Ca^<2+>-channel blocker, charlbdotoxin, specifically inhibited the rapid initiation of sperm motility by JE, suggesting that those channels participate in the initiation of sperm motility caused by the SMIS.Whereas, sperm motility was induced by the decrease of osmolality in the other amphibians, including the primitive species of urodeles, that undergo external fertilization. Sperm of C. pyrrhogaster also begins to move under low osmolality. In this study, when dry sperm were added in water, they rapidly began to move like those in the JE. However, the initiation of sperm motility induced by water was independent of pH within the range of 7 to 8.5 while that induced by SMIS was strengthened under the high pH condition. Furthermore, water-induced sperm motility did not make vigorous within 10 min while most sperm in the JE vigorously moved in 10 min. Two distinct mechanisms for the regulation of sperm motility may be equipped in the sperm of urodeles, which may allow sperm to begin to move under the distinct conditions in the species-specific fertilization modes.

蝾螈进行体内受精。精子直接在雌性泄殖腔内的卵胶表面受精。我们已经报道，大多数精子开始迅速运动的处理卵冻提取物（JE）的蝾螈，cynops pyrrhogaster，和精子运动启动因子（SMIS）的JE是负责精子运动的快速启动。鸡蛋冻还含有阳离子，可以参与调节精子的运动。本研究旨在阐明这些因子在蛋冻生理条件下的作用。根据估计的pH和离子浓度制备重构离子溶液（RIS）。当将干精子加入JE-RIS中时，大多数精子在3分钟内开始运动。K^+通道阻断剂维拉帕米和Ca^<2+>通道阻断剂charlbdotoxin特异性地抑制JE对精子运动的快速启动，表明这些通道参与SMIS引起的精子运动的启动。而在其他两栖动物中，精子运动是由渗透压的降低引起的。包括原始种类的有尾目动物，它们经历外部受精。C.精子在低渗透压下红腹菌也开始移动。在这项研究中，当干燥的精子被加入水中时，它们迅速开始像JE中的精子一样运动。在pH值7 ~ 8.5范围内，水对精子运动的启动作用不受pH值的影响，而SMIS对精子运动的启动作用在高pH值条件下得到加强。此外，水诱导的精子运动并没有在10 min内变得活跃，而在JE中的大多数精子在10 min内活跃地运动。有尾目动物的精子可能具有两种不同的精子运动调节机制，这可能允许精子在种特异性受精模式中的不同条件下开始运动。