Histamine and polyamine changes in tissue development of tongue and drugs effects.

这项研究的目的是阐明组胺和多胺在组织发育中的作用。因此，我们研究了肿瘤细胞移植或DYBA(9，10-二甲基-1，2-苯并茂)诱发舌癌后舌组织中这些胺水平和组氨酸脱羧酶(HDC)活性的变化。采用肥大细胞基因缺陷的W/W^v小鼠，观察艾氏腹水细胞舌内移植后早期肿瘤生长和组胺含量的变化，并与同基因+/+小鼠和正常ddy小鼠的变化进行比较。同时，W/W^v小鼠肿瘤体积小于+/+或ddy。植入后第6天，+/+和ddy舌中组蛋白含量下降，而W/W^v舌中组蛋白含量升高。这些结果表明，肥大细胞和组胺在组织生长过程中起着重要作用，特别是在早期阶段。我们正在使用HDC抑制剂-FMH来研究组胺的作用。在金黄地鼠舌上涂抹DMBA，观察HDC和ODC(鸟氨酸脱羧酶)活性的变化。加入DMBA后，HDC和ODC活性均升高，但当加入放线菌素D时，仅HDC活性显著增强。这些结果表明，DMBA涂抹后HDC活性的快速增加可能是由于诱导了舌非肥大细胞中的“诱导性HDC”。观察了DMBA涂抹诱发的仓鼠舌癌模型中组胺和多胺含量的变化。结果表明，组胺在舌癌的早期发育过程中起重要作用，组胺在舌癌的早期发育中起重要作用。

The aim of this investigation was to clarify the role of histamine and polyamines in tissue development. So, we studied on the changes of these amines levels and activity of histidine decarboxylase ( HDC ) in the tongue tissues after implantation of tumor cells or provocation of tongue carcinoma induced by DYBA ( 9,10-dimethyl-1,2-benzanthracene ).1. W/W^v mice, genetically mast cell deficient, was employed to examine the changes of tumor growth and histamine content in early stages after implantation of Ehrlich ascites cells in the tongue and compared with the changes in those of congenic +/+ mice or normal ddY mice. The tumor volume in W/W^v mice was smaller than +/+ or ddY at the same time. Histamnine contents in the tongues of +/+ and ddY decreased 6 day after implantation but in W/W^v it increased. These results suggest that mast cells and histamine play an important role in tissue growing process, especially in early stages. We are investigating on the histamine roles using -FMH, a HDC inhibitor.2. DMBA was painted on the tongue of golden hamster and changes of activities of HDC and ODC ( ornithine decarboxylase ) were estimated. Application of DMBA induced the increases of HDC and ODC activities, but, when applicated with Actinomycin D, only HDC activity was markedly enhanced. These results suggest that rapid increase of HDC activity by painting with DMBA may be due to the induction of " inducible HDC " in non-mast cells in tongue.3. The changes of histamine and polyamine contents in the hamster tongue carcinoma induced by DMBA painting were estimated. There was significant increase of polyamine contents and X-ray radiation decreased the contents of polyamines, but histamine content was not changed.In this investigated, we got the results suggesting that histamine plays an important role in early stages of tissue development, especially in tumor development in tongue.