Bryotherm - Thermomorphogenesis as a mechanism to adapt to elevated temperatures in early land plant evolution

植物在其整个生命周期中必须忍受的最低和最高环境温度之间的幅度在陆地环境中比在水生环境中要大得多。因此，特别是环境温度的升高是植物成功在陆地上定居所必须应对的最突出的非生物因素之一。众所周知，优树陆地植物的表型可塑性使它们能够调整地上部结构以适应和适应高温。这种机制，即热形态发生，是如何以及何时起源的，目前尚不清楚。同样，我们不知道苔藓植物是否会进行热形态发生，因为苔藓植物的身体结构可能与第一代陆地植物非常相似。因此，在这个项目中，我们将从形态和生理水平上描述苔藓植物(Physcomitrella patens和Marchantia Polya)对高温的响应，包括阐明温度敏感的信号级联。除了比较分析外，我们还将采用公正的方法，主要使用地钱。为了识别信号和响应模块，我们将生成高密度转录和磷酸蛋白质组数据集，并使用它们基于机器学习方法构建基因调控网络。因此，我们的目标是在这个项目中结合形态和分子数据，以更好地了解苔藓植物如何应对环境温度升高。有了这些知识。然后，我们可能能够设计实验，直接解决这种机制的出现及其在植物对土地进行陆地改造过程中的作用。

The amplitude between the minimum and the maximum ambient temperature a plant has to endure during its life cycle is much larger in terrestrial than in aquatic environments. As such, especially elevated ambient temperatures are among the most prominent abiotic factors plants had to cope with to successfully colonize land. It is known that the phenotypic plasticity in eudicot land plants allows them to adjust shoot architecture to acclimate and adapt to elevated temperatures. How and when this mechanism, thermomorphogenesis, has originated is unknown. Likewise, we do not know whether bryophytes, whose body plans is probably very similar to that of the first terrestrial plants, perform thermomorphogenesis. In this project, we will therefore characterize the response of bryophytes (Physcomitrella patens and Marchantia polymorpha) to elevated temperatures on both the morphological as well as the physiological level, including elucidation of temperature sensitive signaling cascades. In addition to comparative analyses we will also follow an unbiased approach, primarily using the liverwort Marchantia polymorpha. To identify signaling and response modules, we will generate high density transcriptomic and phosphoproteomic datasets and use them to construct gene regulatory networks based on machine learning approaches. As such, we aim to combine morphological and molecular data in this project to better understand how bryophytes cope with elevated ambient temperatures. With this knowledge. we might then be in a position to design experiments that directly address the emergence of such mechanisms and their role during the terrestrialization of land by plants.