Studies of Tissue Culture for Mangrove Plants

对红树科几种尚未报道成功的红树林植物进行了愈伤组织诱导研究，并进行了再分化试验。1991 ~ 1992年，在日本冲绳县的伊里表岛和冲绳岛采集了秋茄、红海榄和海榄3种红树林标本。茎尖和根的茎尖也用于愈伤组织的诱导。茎尖和根的茎尖也用于愈伤组织的诱导。植物激素及其浓度分别为2，4-D和激动素，以及这些组合分别为5 × 10 ~（-1）M、<-5>5 × 10 ~（-1）M<-6>和5 × 10 ~（-1）<-7>M。使用的培养基是Muraelum和Skoog以及0.8%琼脂。秋茄的茎尖和根部均不能诱导出愈伤组织。由于这些组织是如此不稳定，受绝育的影响，并在移植后两周内死亡。只有外植体的繁殖体能够识别愈伤组织的形成，特别是未成熟的。研究了红海榄和B.gymnorrhiza的繁殖体的几个不同发育阶段，发现早期和幼嫩的繁殖体均可产生愈伤组织。两种植物成熟繁殖体的外植体即使在严格的灭菌条件下也不能成功地被真菌或细菌污染。3种红树林的顶叶和根的茎段均不能形成愈伤组织，而能从早期或幼嫩阶段的繁殖体中形成愈伤组织。这些事实通过以下几个实验得到了证实。激素处理的浓度也通过相同的实验确定。在2.4-D、IAA和Kinetamine的组合以及上述每三种浓度下进行了红海榄愈伤组织再分化的一些实验。愈伤组织很少分化出不定芽。这种情况正在研究中。

Studies of callus induction for some mangrove species(Rhizophoraceae) were done, that had not been reported to succeed from it. Several experiments of redifferentiation were also done. Propagules of three mangrove species(Kandelia candel, Rhizophora stylosa and Bruguiera gymnorrhiza) used for experiments were collected from lriomote and Okinawa island, Okinawa prefecture, during 1991 and 92. Terminal and root merystem were also used for callus induction. Terminal and root merystem were also used for callus induction. Plant hormone and its concentration were 2,4-D and Kinetin, and these combination of 5^*10^<-5>, ^*10^<-6> and ^*10^<-7>M respectively. Culture media used was Murasige and Skoog and 0.8% agar. Both terminal and root merystem of K.candel were not recognized to induce callus tissue. Because these tissues were so labile that affected by the sterilization and died with in two weeks after transplanting. Only explant of propagule were able to recognize callus formation, especially from immature ones. Several different developing stage of propagule of R.stylosa and B.gymnorrhiza studied, were recognized to produce callus from the early or young ones. Explant from mature propagules of two species were not successful by the contamination of fungi or bacteria even under heavy sterilization. All three mangrove species were not able to form callus from terminal and root merystem, but from the propagules in early or young stage of development. These facts were confirmed by following several experiments. Concentration of hormonal treatments are also determined by the same experiments. Some experiments for redifferentiation from R.stylosa callus were done under combinations of 2.4-D and IAA, and Kinetine, and each three concentrations described above. Few adventituous bud were able to differentiate from callus tissues. This condition are now under examination.