Construction of a nitrite sensor based on biocatalyst electrode

基于生物催化电极的原理，研制了一种亚硝酸盐传感器。细胞色素c等血红素蛋白被用作亚硝酸盐电化学还原的催化剂。研究发现，在酸性溶液中，微量细胞色素c的存在能够在较低的负电势下有效地还原亚硝酸盐。细胞色素c的催化作用即使在10ﾟC中处理30分钟也不变。结果表明，催化活性主要是由于细胞色素c吸附在电极表面所致。利用黄血球蛋白和藜麦血球蛋白，详细研究了吸附的血红素蛋白的电化学行为。结果表明，利用血红素蛋白、细胞色素c、肌红蛋白和血红蛋白可以构建针状传感器。甲基紫精也被发现作为催化剂在电极上还原闪锌矿，在中性溶液中减少了过电位。

A nitrite sensor was developed on the basis of the principle of biocatalyst electrodes. Heme proteins such as cytochrome c was employed as a catalyst to reduce nitrite electrochemically.1. It was found that the presence of a trace amount of cytochrome c was effective to reduce nitrite at less negative potentials in acidic solutions.2. The catalytic effect of cytochrome c remained unchanged even when treated at lOO ﾟC for 30 min.3. It was revealed that the catalytic activity was due to cytochrome c adsorbed on the surface of electrodes.4. The electrochemical behavior of adsorbed heme-proteins was studied in detail by the use of flavohemo-proteins ans quinohemo proteins.5. It was shown that nitite sensors could be constructed by usisng heme proteins, cytochrome c, myoglobin and hemeglobin.6. Methyl viologen was found to be also effective as a catalyst to reduce nitite at electrodes with a reduced overpotential in neutral solutions.