Regulation of gene expression of cholecystokinin in rat intestin e and mechanim or its release.

1.建立了胆囊收缩素（cholecystokinin，CCK）和促胰液素（secretin，促胰液素）mRNA水平的检测体系，分别用CCK的343 bp cDNA（正义引物5 '-AGCCGGTAGTCCCTGTAGAA-3'和反义引物5 '-GTGCGTGGTTGTTTTCTCAT-3'）和促胰液素的426 bp cDNA（正义引物5 '-ATGGAGCCTCTACTGCCCACGC-3'和反义引物5 '-AGGGAGGAAGGGAGTCTCCAG-3'）进行RT-PCR.胆汁和胰液的排除显着增加血浆CCK浓度，峰值在1-2小时后转移。胆胰液的阻断也促进了CCK和促胰液素基因的表达，并在术后4 h达到高峰.胆胰液分流可显著提高血浆和小肠CCK浓度、肠粘膜CCK mRNA水平和胰腺PSTI。PSTI-61的mRNA水平的增加高于PSTI-56。CCK拮抗剂可抑制这些变化，但CCK激动剂不能完全复制这些变化.胆囊收缩素mRNA水平在胰管结扎后第1天开始下降，然后上升，第10天达到高峰，第14天略有下降，第28天恢复到对照组水平。PSTIs的mRNA水平在缺血后1、3、7、10 d显著升高。PAP的mRNA水平在第1天和第3天出现，第1天增加最多。PAP基因表达的变化最敏感地反映胰腺的急性炎症变化。

1. We established the assay system of mRNA levels of cholecystokinin (CCK) and secretin, using the TR-PCR products of 343 bp cDNA (the sense primer 5'-AGCCGGTAGTCCCTGTAGAA-3' and anti-sense primer 5'-GTGCGTGGTTGTTTTCTCAT-3') for CCK and 426 bp cDNA 9the sense primer 5'ATGGAGCCTCTACTGCCCACGC-3' and anti-sense primer 5'-AGGGAGGAAGGGAGTCTCCAG-3') for secretin, respectively.2. Exclusion of bile and pancreatic juice significantly increased plasma CCK concentrations, peaking at 1-2 h after diversion. Excludion of bile and pancreatic jucie also enhanced gene expressions of both CCK and secretin, peaking at 4 h after diversion.3. The concentrations of plasma and intestinal CCK, and the levels of mRNA of CCK in the intestinal mucosa and PSTIs in the pancreas were significantly increased by the bile-pancreatic juice diversion. The increase in the level of mRNA for PSTI-61 was igher than that for PSTI-56. CCK-antagonist inhibited these changes but administration of a CCK agonist could not fully reproduce them.4. The CCK mRNA level initially decreased on day 1 and then increased and peaked on day 10, slightly decreased on day 14 and returned to the control level on day 28 after ancreatic duct lgaton. The level of mRNA of PSTIs increased significantly on days 1, 3, 7, 10 after occlusion. The mRNA level of PAP appeared on days 1 and 3, and the maximal increase was observed on day 1. The changes of gene expression of PAP reflects the acute inflammatory changes of ancreas most sensitively.