Gene expression and gene polymorphisms related with chole-pancreatic diseases

我们发现基因多态性与胆胰疾病呈正相关。 CCK-A 受体 (AR) 基因多态性（-81A/G、-128G1T）与恐慌症、酗酒和肥胖有关。肾上腺素β3R突变（Trp64Arg）与较高的BMI相关，而ALDH2突变（Glu至Lys）与吸烟者患胰腺癌的风险有关。生长素释放肽受体多态性（177T/C）是神经性贪食症的一个风险。 CEL基因多态性与酒精性胰腺炎相关。 SHIP2基因多态性与葡萄糖耐受不良相关。但未发现西部地区已报道的PAR2、Klotho、Cyp2A6和Ghrelin多态性。胆囊结石时CCK-AR基因表达降低。但CCK-AR多态性与CCK-AR基因表达水平是否存在正相关尚不清楚。我们观察到了CCK-ARKO小鼠的新表型。 CCK-ARKO小鼠在受到束缚应激后表现出暴饮暴食，并且比其他基因型小鼠饮酒更多，这可能是由于伏隔核中多巴胺2R蛋白的表达较高。与野生型小鼠相比，CCK ARKO 小鼠胆结石形成的频率增加，但性别差异尚不清楚。

We found positive associations of gene polymorphisms and chole-pancreatic disorders. The CCK-A receptor (AR) gene polymorphism (-81A/G, -128G1T) was associated with panic disorder, alcoholism, and obesity. The adrenalin beta 3R mutation (Trp64Arg) was associated with higher BMI, and ALDH2 mutation (Glu to Lys) was the risk of pancreatic cancer in the smokers. The ghrelin receptor polymorphism (177T/C) was a risk of Bulimia Nervosa. The CEL gene polymorphism was associated with alcoholic pancreatitis. The SHIP2 gene polymorphism was associated with glucose intolerance. However, we could not found polymorphisms of PAR2, Klotho, Cyp2A6, and Ghrelin, which had been reported in the Western counties.The gene expression of CCK-AR was decreased in the gallbladder with gallstones. However, it is still unknown whether there were positive associations of CCK-AR polymorphism and the levels of CCK-AR gene expression.We observed new phenotypes of CCK-ARKO mice. CCK-ARKO mice showed over-eating after the restraint stress, and they drank alcohol more than other genotypes, possibly due to the higher expression of dopamine 2R protein in the nucleus accumbens. The frequency of gallstone formation was increased in CCK ARKO mice, compared with wild-type mice, however, the sex difference was not clarified, yet.