Immunohistochemical Study of Glutathione-related Enzyme and Proliferative Antigen in Non-Small Cell Lung Cancer

对107例非小细胞肺癌患者切除肿瘤组织，检测谷胱甘肽过氧化物酶（GPX）、谷胱甘肽还原酶（GR）、增殖细胞核抗原（PCNA）、表皮生长因子受体（EGFR）表达与CDDP敏感性的关系。肿瘤细胞在CDDP溶液中孵育后，通过DNA直方图从s期或g2m期细胞的增加来评估CDDP敏感性。采用间接免疫过氧化物酶法检测各组肿瘤组织中GPX、GR、PCNA和EGFR的表达。根据组织学类型，对CDDP敏感的患者比例在大细胞癌中为40%，鳞状细胞癌为31%，腺癌为6%。GPX和GR表达频率与CDDP敏感性呈负相关。cddp敏感组GPX和GR表达明显低于cddp耐药组。而在非小细胞肺癌中，敏感组PCNA和EGFR的表达明显降低。从上述发现来看，对这些抗原进行免疫组化研究可能有助于预测非小细胞肺癌的CDDP敏感性。

With resected tumor tissue from 107 patients with non-small cell lung cancer, the expression of glutathione peroxidase (GPX), glutathione reductase (GR), proliferating cell nuclear antigen (PCNA), and epidermal growth factor receptor (EGFR) was examined in relation to CDDP sensitivity. The CDDP sensitivity was assessed from an increase of cells in the S-phase or G2M-phase by a DNA histogram after the tumor cells were incubated in a CDDP solution. The expression of GPX,GR,PCNA and EGFR for each tumor was studied with an indirect immunoperoxidase technique on paraffin-embedded tissues.The percentage of patients sensitive to CDDP according to the histologic type was 40% for large cell carcinomas, 31% for squamous cell carcinomas, and 6% for adenocarcinomas. There was an inverse relationship between CDDP sensitivity and the frequency of GPX and GR expression. The GPX and GR expression was significantly lower in the CDDP-sensitive group than in the CDDP-resistant group. However, the expression of PCNA and EGFR was significantly lower in the sensitive group in non-small cell lung cancer.From the above findings, an immunohistochemical study of these antigens may be useful for predicting CDDP sensitivity in non-small cell lung cancer.