The study on X-Y spirmpreseparation and Testie determining gene

性连锁遗传病的治疗方法是配子阶段的预选和早期胚胎的选择。本研究的目的是发展精子预选程序，建立精液选择前DNA探针分析测定精子X和Y的方法。用荧光原位杂交(FISH)方法证实了X和Y精子的分布。建立了精子核的解凝程序。精子核必须用二硫苏糖醇(DTT)处理，才能使DNA探针穿过核膜。DXY-1探针和DYZ-1分别被用作X和Y精子的特异性DNA探针。为了减少所需的精子数量，精子性别分析的发展方向是采用聚合酶链式反应扩增方法。性别决定损伤Y(SRY)基因用于确定精子的存在。虽然最终的目标应该是单个精子细胞的分析，但它仍然是精子细胞数量减少的过程。

The patients of sex-linked genetic deisorders have been disired to be curedby the teatment of preselection at the stage of gametes and the selection at the early embryos. Specially the objective of this study is the development of sperm preselection procedures and establishment of the detemination method for sperm X and Y using DNA probe analysisi prior to its selection. The distribution of X and Y sperm has been demonstrated by fluorescence in situ hybridization (FISH) nethod. The decondensation precedure of the sperm nuclei was established. The sperm unclei have to be treated with diethiothreitol (DTT) for getting DNA probe through nuclear membrane. DXY-1 probe and DYZ-1 have been used as specific DNA probe for X and Y sperm respectively. The development of sperm sex analysis has been directed to PCR amplification method for the purpose of reducing necessary numbers of sperm cells. The sex determinig lesion Y(SRY) gene was used to determine the existence of sperm. although final objective should be the analysisi from single sperm cell, it is still of the reducing process of sperm cell umbers.