Evaluation Of Clinical Laboratory Test By Using Quartz Crystal Resonator

研究了用石英晶体粘度传感器测定血浆纤维蛋白原浓度的方法。采用AT-Cut晶体作为粘度传感器，在液相中采用井型晶胞。在石英晶体表面预涂一层纤维蛋白原以保护内源性纤维蛋白原在血浆中的结合，通过在反应混合物中加入核黄素来启动检测，利用井型池、振荡器和频率计数器监测共振频率的变化。由于纤维蛋白原凝胶化为纤维蛋白而导致粘度增加，共振频率降低。该方法简便、快速、专属、准确，检测范围在50~400 mg/dl范围内线性关系良好。与常规方法比较，Y=110.21(n=20)，相关系数r=0.702，Y=1.89X(建议方法)。重复性(CV)在2.29%~7.97%之间。初步研究表明，石英晶体谐振器可用于测定血浆凝血因子在液相中的凝胶化程度。

Quantification of plasma fibrinogen concentration by quartz crystal viscosity sensor was investigated. At-cut crystal was used as viscosity sensor with a well-type cell in a liquid phase. The surface of quartz crystal was precoated with fibrinogen to protect the bonding of endogeneous fibrinogen in the plasma.The assay was initiated by the addition of thrambin into reaction mixture.The resonant frequency change was monitored by using a well-type cell, oscillator and frequency counter. The resonant frequency decrease due to the increase of viscosity by gelation of fibrinogen converted to fibrin.The proposed method is simple, rapid, specific and accurate at the lower range.Results obtained in proposed system showed good linearity over range 50-400 mg/dl. The comparison of proposed method with routine method used yielded a correlation coefficient ; gamma=0.702 and regression curve ; Y=1.89X(proposed method) - 110.21(n=20).Reference interval of plasma fibrinogen concentration from healty adults has in the range of 170-390 mg/dl. Reproducibilities(CVs) was between 2.29% and 7.97% in plasma. The preliminary studies suggested that a quartz crystal resonator is useful for the measurement of gelation of plasma coagulation factors in the liquid phase.