Fluorescence Determination of Optical Purity of Aminoacid Ester using Fluorescence Labeled Chiral Host Molecules

为了开发一种新的测定手性化合物光学纯度的荧光技术，合成了手性杯[4]芳烃标记的荧光基团(芘)作为荧光指示剂，与氨基酸醚衍生物相互作用。在手性柱的辅助下，用高效液相色谱对荧光手性杯[4]芳烃进行了光学拆分。合成的杯芳烃的旋光性由化合物的圆偏色度测定所证实。尽管荧光手性杯[4]芳烃与氨基酸醚衍生物的络合反应不是很灵敏，但本研究为利用荧光主体分子作为手性指示剂测定手性化合物的光学纯度提供了基础。

In order to develop a new fluorescence technique for the determenation of optical purity of chiral compounds, chiral calix [4] arenes labeled fluorescent moieties (pyrene) were synthesized as fluorescent indicators which interact with aminoacid ether derivatives. Optical resolution of the fluorescent chiral calix [4] arenes were carriedout by using HPLC with the aid of a chiral column. Optical activity of thesynthesized calixarenes were confirmedby the measurement of circular dechrorism of the compounds. Although the fluorescent chiral calix [4] arenes does not exhibit high sensitivities in the complexation with aminoacid ether derivatives, this research provides a basicidea for the determination of optical purity of chiral compounds using fluorescent host molecules as chiral indicators.