Isolation and Molecular Phyogeny of the Most primitive Eukaryotes in Algae

1994年，从栃木县汤本温泉那须的酸性温泉中分离到单细胞藻类，Cyanidium caldarium和Galdieria sulphuraria。1995年在日本伊豆半岛的热川温泉、矿山温泉、修善寺温泉等中性温泉中发现了蓝绿藻、聚球藻、席藻和颤藻等。其基因部分序列与其它红藻有较大差异。我们推测，C.caldarium可能远不是标准的红藻。

Unicellular algae, Cyanidium caldarium and Galdieria sulphuraria were isolated from a acid-hot springs, Nasu, Yumoto spa, Tochigi prefecture in 1994. Blue-green algae (Cyanobacteria), Synechococcus, Phormidium and Oscillatoria were found in hot-springs at neutral pH, such as Atagawa, Mine and Shuzenji spas, Izu peninsula in 1995.Then, total DNA were extracted from C.caldarium, G.sulphuraria and C.merolae and 18s rRNA genes of them were multiplied by using PCR.As a result, the gene was much obtained from C.caldarium. Partial DNA sequence of the gene of C.caldarium are considerably different from those of other red algae. We postulate that C.caldarium may be far from standard red algae phylogenetically.