Fundamental Study on Bacterial Cellulose Production

对木质素磺酸盐对细菌纤维素产量的影响进行了两年的研究，结果如下：(1)通过乙醇-水沉淀法对木质素磺酸盐样品进行分级，研究了木质素磺酸盐产量的提高与木质素磺酸盐相对分子质量的关系。结果表明，较高的相对分子质量分数是有效的。(2)在不同的原种培养和碳源条件下证实了添加木质素磺酸盐的增产效果。(3)通过电子显微镜观察发现，添加木质素磺酸盐的培养基中纤维的形成与标准培养基相一致，木质素磺酸盐的增产作用对微细纤维的形成没有任何干扰，这表明木质素磺酸盐的高产机理研究将为细菌纤维素的商业化生产奠定基础。筛选出具有较高纤维素生产能力的菌种可能是实现工业化生产的关键之一。

The effect of lignosulfonate on the bacterial cellulose production has been studied for two years and the following are the results ;(1) The relationship between the high cellulose yield increase and the molecular weight of lignosulfonate was investigated through the fractionation of a lignosulfonate sample by the ethanol-water precipitation method. The result showed relatively high molecular weight fraction is effective.(2) The yield increase by lignosulfonate addition to the medium was confirmed with various stock cultures and carbon sources. The effect of the lignosulfonate had so-called universality.(3) The observation through the transmit electron microscopy showed that the fibril formation by the lignosulfonate added medium is as normal as the standard medium, and the yield increase effect of lignosulfonate occured without any interference to the micro-fibril formation.Above resutlts indicate that the reasearch on the mechanism of the high yield increase of lignosulfonate addition will lead to a fundamental condition for the commercial production of bacterial cellulose. The screening of bacteria with a high cellulose procuction ability might be one of other essentials for the commercial use.