Immunologic significance of intraepithelial lymphocytes in human palatine tonsils : Morphofunctional approach using immunoelectron microscopy and labeled antigen method

覆盖人腭扁桃体的网状粘膜的特征在于主要由B细胞和浆细胞组成的单核细胞的致密浸润和毛细血管网络的存在。浆细胞主要分布于网状粘膜的基底部。粘膜B细胞有4种类型：bcl-2阳性、CD 10/CD 23阴性的“边缘带”型，sIgD/CD 5/CD 24阳性的“套带”型，CD 10/DNA 7阳性的“生发中心”型，CD 11 b阳性、CD 16阴性的“B-1”型。免疫电镜双染鉴定CD 5阳性B细胞。T细胞主要分布在粘膜中，以CD_4阳性为主，同时也有少量的胸腺外T细胞分布于粘膜中，这些T细胞可能为CD_（56）和TCR γ δ阳性。对于“B-1”型B细胞和“胸腺外”T细胞等遗传上古老的淋巴样细胞的功能意义还需进一步研究。扁桃体和胸腺髓质的网状粘膜之间的形态功能相似性是值得注意的。在制备MBP标记的融合蛋白用于“标记抗原法”以证明细胞产生流感嗜血杆菌特异性抗体时遇到了困难。使用细菌DNA的随机标记不成功。然后，我们尝试通过PCR方法制备含有流感嗜血杆菌特异性蛋白质片段如蛋白质D和脂肪酸结合蛋白的重组蛋白质，但目前尚未获得令人满意的结果。将继续努力建立这种新方法的标记探针的方法。

The reticular mucosa covering the human palatine tonsils was characterized by the dense infiltration of mononuclear cells mainly consisting of B-cells and plasma cells and by the presence of the capillary network. The plasma cells predominantly containing IgG were distributed in the basal half of the reticular mucosa. Four kinds of B-cells were observed in the mucosa, including 1) bcl-2-positive and CD10/CD23-negative "marginal zone" -type, 2) sIgD/CD5/CD24-positive "mantle zone" type, 3) CD10/DNA7-positive "germinal center" type, and 4) CD11b-positive and CD16-negative "B-1" type. CD5-positive-B-cells were identified by double staining in immunoelectron microscopy. As for T-cells, CD4-positive T_H cells predominated in the mucosa, where a few putative extrathymic T-cells positive for CD56 and TCRgammadelta were distributed. Furtherstudies are required to clarify the functional significance of the phylogenetically old lymphoid cells, such as "B-1" type B-cells and "extrathymic" T-cells. Morphofunctional similarities between the reticular mucosa of the tonsil and the medulla of the thymus were noteworthy.Difficulties were encountered in the preparation of MBP-labeled fusion proteins for the "labeled antigen method" to demonstrate cells producing antibodies specific for Hemophilus influenzae. Random-labeling using the bacterial DNA was unsuccessful. Then we tried to prepare recombinant proteins containing fragments of H.influenzae-specific proteins such as protein D and fatty acid-binding proteins by using a PCR method.However, we do not yet obtain satisfactory results at present. Continuous effort will be made for the establishment of the method for labeled probes for this new approach.