Expression of human endogenous retrovirus gene in tumor cells

人内源性逆转录病毒基因整合在染色体DNA中，由有序的转录因子表达。我们研究了人内源性逆转录病毒基因Clone 4-1的启动子活性。以荧光素酶为报告基因，以Jarkat细胞为研究对象，确定了Clone 4-1 LTR中的启动子片段，确定了184-BP片段具有启动子活性，242-BP片段具有抑制表达活性。通过凝胶移位实验和体内足印实验，观察到DNA结合蛋白的存在。我们的研究表明，人内源性逆转录病毒基因的表达是由一种少量的DNA结合蛋白作为转录因子参与这一过程。

Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.