Expression of interleukin-19 in synovial tissue from patients with rheumatoid arthritis

探讨白细胞介素-10在类风湿关节炎（RA）中的表达及其致病作用。与骨关节炎患者相比，RA患者血清和滑液中IL-10的浓度更高。RA滑膜组织IL-10 mRNA的表达强于OA，并自发产生更高水平的IL-10。通过免疫组化染色和分离的RA滑膜巨噬细胞、成纤维细胞和T细胞IL-10的检测，内层和亚内层的巨噬细胞都是这种细胞因子的主要来源。IL-10明显抑制RA滑膜产生炎性因子IL-1和TNF-α，但这两种细胞因子是IL-10的强诱导剂。另一方面，T细胞衍生的抗炎细胞因子IL-4和Il-13在抑制滑膜炎症细胞因子方面效率较低，但能够抑制RA滑膜成纤维细胞的增殖。然而，它们在RA关节中的表达非常有限，IL-10的表达与RA中igg -类风湿因子的水平相关。这些结果表明，IL-10可能主要负责RA中慢性炎症和类风湿因子产生的下调，尽管其滑膜表达不足以控制疾病活动。在IL-6型细胞因子中，大量的IL-6、IL-11和LIF主要由IL-1或tnf活化的成纤维细胞产生，而肿瘤抑制素M仅由RA关节的巨噬细胞产生。然而，只有IL-6的循环水平与血清CRP水平升高相关，表明其参与全身炎症反应，如肝脏产生急性pahse反应物。

The expression of interleukin-10 and its pathogenic role in rheumatoid arthritis (RA) was investigated. Concentrations of IL-10 in both serum and synovial fluid from RA patients were greater compared to those of osteoarthritis patients. Synovial tissues in RA more strongly expressed IL-10 mRNA than in OA, and spontaneously produced higher levels of IL-10. By Immunohistochemical staining and detection of IL-10 in isolated macrophages, fibroblasts, and T cells from RA synovium, macrophages in both the lining and sublining layer are major sources of this cytokine. IL-10 markedly inhibited the production of inflammatory cytokines such as IL-1 and TNF-α by RA synovium, but both cytokines were potent inducer of IL-10. On the other hand, T cell-derived anti-inflammatory cytokines, IL-4 and Il-13, are less efficient in inhibiting synovial inflammatory cytokines, 7but are able to inhibit proliferation of RA synovial fibroblasts. However, their expression in RA joint is extremely limited IL-10 expression was associated with levels of IgG-rheumatoid factor in RA. These results indicate that IL-10 may be mostly responsible for downregulation of chronic inflammation and rheumatoid factor production in RA, although its synoival expression if not sufficient to control the disease activity.Of IL-6-type cytokines, large amounts of IL-6, IL-11, and LIF were produced mainly by IL-1 or TNF-activated fibroblasts, whereas oncostatin M was exclusively by macrophages in RA joints. However, only IL-6 was circulating at significant levels in association with raised levels of serum CRP levels, indicating its contribution to systemic inflammatory reactions such as acute-pahse reactant production by liver.