An experimental study of enhanced anticancer effect of intraperitoneal chemotherapy with high energy shock waves

腹腔注射顺铂的体外抗肿瘤活性进行了研究。将Erhlich腹水癌细胞悬浮在含有5-50 μ g/ml顺铂的生理盐水中30分钟，并测量顺铂的细胞内摄取。Erhlich细胞摄取的顺铂蓄积与顺铂浓度成比例增加。在10 μ g/ml顺铂溶液中顺铂的细胞摄取按时间顺序增加。在此基础上，研究了顺铂腹腔内给药和体外低强度超声波的体内效应。通过腹腔注射Erhlich细胞在ddy小鼠中诱导腹膜癌病。在细胞注射后24和94小时，将顺铂溶液（50 μ g/ml）以5 mg/kg的剂量给予ddy小鼠。超声波（28 kHz）照射小鼠3、5或10分钟。注射细胞后24小时治疗组小鼠的中位生存期分别为11.5、14.5、14.0、21.5和23天，对照组小鼠的中位生存期分别为11.5、14.5、14.0、21.5和23天， ...更多信息 单独的顺铂、3分钟的超声波、5分钟的超声波和10分钟的超声波。腹腔内顺铂联合5分钟或10分钟超声波治疗能显著延长荷瘤ddy小鼠的生存时间（P<0.01）。注射细胞后96 h处理，未处理组、顺铂组、超声波3 min组、超声波5 min组和超声波10 min组的中位生存天数分别为12.0、16.0、15.5、22.0和24.5。腹腔内顺铂和5分钟或10分钟超声波治疗能显著延长荷瘤鼠的生存期（P<0.01）。这些结果表明，腹腔内顺铂和体外低强度超声波联合使用可能比单独腹腔内顺铂治疗更有效地预防和治疗腹膜播散。少

Antitumor activity of intraperitoneal cisplatin was examined in vitro. Erhlich ascites carcinoma cells were suspended in phyisiolosic saline which contained 5-50 mug/ml of cisplatin for 30 minutes, and intracellular uptake of cisplatin was measured. The accumulation of cisplatin taken up by Erhlich cells increased in proportion to the concentration of cisplatin. The cellular uptake of cisplatin in 10 mug/ml cisplatin solution increased chronologically. Then in vivo effects of intraperitoneal cisplatin and extracorporeal low-level ultrasonic waves were studied. A peritoneal carcinomatosis was induced in ddy mice by intraperitoneal injection of Erhlich cells. At 24 and 94 hours after cell injection, cisplatin solution (50 mug/ml) was administered at a dose of 5 mg/kg to ddy mice. Ultrasonic waves (28 kHz) were irradiated to mice for 3,5, or 10 minutes. When mices were treated at 24 hours after cell injection, the median survival days were 11.5,14.5,14.0,21.5 and 23 in the no-treatment, c … More isplatin alone, 3-minute ultrasonic waves, 5-minute ultrasonic waves and 10-minutes ultrasonic waves, respectively. The survival of intraperitoneal tumor-bearing ddy mice was greatly prolonged by intraperitoneal cisplatin and 5- or 10-minute ultrasonic waves as compared with those of the no-treatment or cisplatin aalone (P<0.01, Cox-Mantel test). When mices were treated at 96 hours after cell injection, the median survival days were 12.0 16.0,15.5,22.0 and 24.5 in the no-treatment, cisplatin alone, 3-minute ultrasonic waves, 5-minute ultrasonic waves and 10-minutes ultrasonic waves, respectively. The survival of intraperitoneal tumor-bearing ddy mice was greatly prologned by intraperitoneal cisplatin and 5- or 10-minute ultorasonic waves as compared with those of the no-treatment or cisplatin alone (P<0.01, Cox-Mantel test). These results indicate that the combination of intraperitoneal cisplatin and extracorporeal low-level ultrasonic waves may prevent and treat the peritoneal dissemination more effectively than the treatment of intraperitoneal cisplatin alone. Less