Detection of the indicator of disease activity in gingival crevicular fluid

目的：确定龈沟液（GCF）中含有什么因子可能是牙周炎疾病活动性的预测因子是非常重要的。因此，我们研究了慢性和急性牙周袋GCF样品中GCF体积和几种炎症因子数量的随时间变化。材料和方法：exp1我们选择了3例表现为成人牙周炎的患者。每例患者选择慢慢期牙周袋2个，要求* 5mm。在基线和13天后，测量附着水平（AL）、探探深度（PD）、探探出血（BOP）。实验期间每隔2 d采集6个牙周袋GCF标本。exp2 . 4例患者均表现为严重牙周炎，且有一个部位出现急性炎症。在基线和4周后进行临床测量，并从这4个周期收集GCF样本。测定GCF的体积和几种炎症因子（il -1 β、PGE_2、总胶原酶）的含量。结果与讨论：慢性期牙周袋临床资料无变化，但实验期间牙周袋内GCF体积及GCF中几种炎症因子的数量随时间发生变化。这种变化的范围在每个口袋之间是不同的，并且无法检测到GCF体积与几种炎症因子的数量之间的相关性。这些结果表明，在慢性期部位，局部炎症反应也在短时间内发生改变。急性期牙周袋区GCF体积及几种炎症因子的数量均明显高于慢性期袋区，随急性炎症程度的减轻而随时间的变化而减少。结果表明急性期局部炎症反应明显增强。然而，由于AL在实验期间没有发生变化，因此不能证明这些可能是疾病活动性的指标。少

Purpose : It is very important to determined what factor containing in gingival crevicular fluid (GCF) may be a predictor disease activity in periodontitis. So, we investigated that time-dependent changes of the volume of GCF and the amounts of several inflammatory factors in GCF samples collected from periodontal pockets in chronic phase and acute phase. Material and Methods : Exp.1 We used 3 patients who demonstrated adult periodontitis. 2 periodontal pockets, required to be * 5mm, in chronic phase were selected from each patient. At baseline and at after 13 days, attachment level (AL), probing depth (PD), bleeding on probing (BOP) were measured. GCF samples were collected from these 6 periodontal pockets at 2 days intervals in experimental period. Exp.2 4 patients who demonstrated severe periodontitis and have a site with acute inflammetion on time, were used. At baseline and at after 4 weeks, clinical measurements were performed, and GCF samples were collected from these 4 periodon … More tal pockets, weekly in experimental period. and the volume of GCF and the amounts of several inflammatory factors (IL-1beta, PGE_2, total collagenase) in GCF samples were measured. Results and Discussion : In periodontal pockets in chronic phase, clinical data had no change, but the GCF volume and the amounts of saveral inflammatory factors in GCF time-dependently changed within experimental period. The range of this changes were different between each pocket, and the correlation between GCF volume and the amounts of several inflammatory factors could not be detected. these results suggest that also in sites of chronic phase, local inflammatory responses were changed within short time. In periodontal pokets in acute phase, the GCF volume and the amounts of several inflammatory factors in GCF,which were much higher than that in pockets in chronic phase, time-dependently reduced according to the decrease of acute inflammation. This result shows that local inflammatory responses were clearly enhanced in sites of acute phase. However, it could not be proved that these may be the indicator of disease activity, because AL was not changed within experimental peiod. Less