Basic experimental study for clinical application of osteoblastic cells and the produced extracellural matrix as a biomaterial.

将冻融细胞膜植入小鼠肌肉组织，验证了成骨细胞作为生物材料应用的实验研究结果。并用免疫组织化学技术证实了该材料具有骨诱导活性。根据这些结果，植入材料在植入后14d钙化后，以膜性成骨为主的成骨过程是进行的。现在我们已经提交了这些结果，并通过植入小鼠皮下检测了人工关节胶原膜和成骨细胞的复合材料。这一概念是用于膜材料的引导组织再生(GTR)系统。结果表明，植入后28天，该复合材料在皮下钙化。这些都支持了该复合材料是用于GTR系统的有用材料。关于转化生长因子-β、胰岛素样生长因子-1和血小板衍生生长因子对成骨细胞生物学影响的研究已经有了一些结果。我们一直在努力制作一份手稿，现在。

The results of experimental study for the application of osteoblastic cells as a biomaterial were examined with the implantation of freeze-thaw cellural film into mouse muscle tissue. And this material has a osteoinductive activity were confirmed with the immunohistochemical technique. According these results, the osteogenesis as membranous ossification is progress after calcification of implanted materials 14days post-implantation. We have been to submit of these results, now.And the complex materials of atelocollagen membrane and osteoblastic cells was examined with the implantation into mouse subcutaneous. This concept is for a membraneous materials of guided tissue regeneration (GTR) system. In the results, this complex materials were calcified in subcutaneous after 28 days post-implantation. These are supporting that this complex material is useful material for GTR system. This series of examination was published, already.And more, we had some results about the biological influences of transforming growth factor-beta, insulin-like growth factor-1 and platelet-derived growth factor to osteoblastic cells, already. We have been trying to make a manuscript, now.