The experimental study of maxillary reconstruction using Bone Morphogenetic Protein (BMP).

生物材料是上颌骨重建所必需的两个概念：一是用成骨材料填充骨缺损区，二是保留骨缺损的空间，使其具有诱导成骨的能力。本研究的目的是用膨化聚四氟乙烯(EPTFE)贴片和骨形态发生蛋白(BMP)复合膜作为骨膜替代物，研究实验骨腔的成骨能力以及生物材料与宿主骨之间的界面。在最初的计划中，福曼BMP被应用于这种复合材料，但它的极化并没有进展。从牛骨基质中提取一毫克BMP用于初步实验研究。BMP被应用于ePTFE贴片的表面。取日本大白兔股骨周围骨膜，制成直径5 mm的钻孔，覆盖ePTFE-BMP复合膜。另一侧也用ePTFE单独作为对照。分别于植入后2周和4周处死动物，取标本进行组织学观察。结果表明，ePTFE-BMP复合膜组的骨缺损区充填了与骨髓混合的新骨组织，膜内侧的微孔充填了新骨。单纯ePTFE组也观察到相同的新骨形成。结果表明，ePTFE-BMP复合膜与单纯ePTFE组无明显差异。

Two concepts of biomaterials are necessory for the maxillary reconstruction : one is to fill the bone defect by osteogenetic materials, and second is to keep the bone defect space for which the former material would be able to induce the osteoinductivity.The aim of this study is to investigate the os Resity of the experimental bone cavity and the interface between biomaterials and host bone using Expanded polytetrafloroethylene (EPTFE) Patch^<TM> composite with Bone morphogenetic protein (BMP) composite (EPTFE-BMP) membrane as a periosteum substitute. At the initial planning, fuman BMP was applied with this composite material, however, polification of it has noe been advanced.One mg of BMP extracted from bovine bone matrix was used for preliminary experimental study instead of fuman BMP.BMP was applied with the surface of the EPTFE Patches. Drill hole of 5mm in diameter was made with removal of surrounding periosteum in the femur of Japanese white rabbit and covered with EPTFE-BMP composite membrane. EPTFE alone was also used as a control on the other side. Two week and four week after implantation, all animals were sacrificed and the specimens were observed histologically. The results demonstrated that bone defect of the EPTFE-BMP composite membrane group was filled with new bone tissue mixed with bone marrow, and micro pores of the inner portion of the membrane were filled with new bone. Same new bone formation was also observed on the EPTFE alone group. The result demonstrated that there was no difference between EPTFE-BMP composite membrane and EPTFE alone group.