Molecular mechanisms of tumorigenesis in primary aldosteronism

本研究的主要目的是利用RNA-seq比较按结节直径和基因型分层的醛固酮生成腺瘤（APAs）的转录谱，来描述导致APAs肿瘤生长失调的分子机制。其次，我们将利用原位基质辅助激光解吸/电离质谱成像（MALDI-MSI）对PA中肾上腺病变的代谢物进行分析，研究醛固酮产生细胞簇向APA过渡的肿瘤发生机制。我们工作的长期目标是改善PA的诊断和管理，以符合慕尼黑PA小组的转化研究目标。

The primary objective of this proposal is to delineate the molecular mechanisms that lead to dysregulated tumor growth of aldosterone-producing adenomas (APAs) using RNA-seq to compare the transcriptional profiles of APAs stratified by nodule diameter and genotype. Secondly, we will investigate the mechanism of tumourigenesis of aldosterone-producing cell cluster to APA transition by metabolite profiling of adrenal lesions in PA using in situ matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). The long term aim of our work is to improve the diagnosis and management of PA in line with the translational research goals of the PA group in Munich.