PPR proteins as modulators of chloroplast translation and RNA stability during temperature acclimation (A07)

PPR蛋白SVR7和PGR3是重要的核糖体生物发生因子，而MRL1是Rubisco表达所必需的PPR。我们发现，这三个PPR基因都是在冷驯化过程中被诱导的，而且总的来说，核糖体生物发生因子和核糖体成分都是受冷调节的。我们的目标是了解在温度适应过程中，所选择的PPR蛋白如何影响RNA的稳定性及其目标mRNAs的翻译。此外，我们将用超分辨率显微镜研究核糖体在叶绿体中发生的生物发生，并将使用定量生物化学来确定核糖体数量如何随着温度驯化的反应而变化。

The PPR proteins SVR7 and PGR3 are important ribosome biogenesis factors and MRL1 is a PPR required for Rubisco expression. We found that all three PPR genes are induced during cold acclimation and that in general, ribosome biogenesis factors and ribosomal components are cold-regulated. Our goal is to understand how the selected PPR proteins influence RNA stability and translation of their target mRNAs during temperature acclimation. Furthermore, we will investigate by super resolution microscopy, where ribosome biogenesis takes place in chloroplasts and will use quantitative biochemistry to determine how ribosome numbers change in response to temperature acclimation.