Fundamental theoretical and experimental investigations of wavefront shaping deep in biological media

所要求的项目的目的是对散射、特别是生物介质中的波前成形及其应用进行基本研究，这些研究基于麦克斯韦方程和辐射传递方程的精确和有效的解析和数值解。例如，由于波前成形引起的聚焦将针对脉冲和连续波源的散射介质中的所有光学性质和聚焦深度来系统地表征。此外，各种技术，例如，反射的弹性散射光和反射的单光子和多光子荧光的相关性研究，将被调查，以实现一个成功的非侵入性的反馈关于形成一个尖锐的焦点深在散射介质。将通过对光学和几何学、部分显微镜下、准确表征的静态和非静态体模以及动物离体组织进行测量来验证理论结果。如果成功的话，这项研究工作将有一系列非常重要的应用，特别是生物组织的显微成像比今天可能的更深。

The aim of the requested project is the fundamental investigation of wavefront shaping deep in scattering, especially biological, media and of its applications based on exact and efficient - both analytical and numerical - solutions of Maxwell's equations and of the radiative transfer equation. For example, the focusing due to wavefront shaping will be characterized systematically regarding all optical properties and the focus depth in the scattering medium both for pulsed and for continuous wave sources. Further, a variety of techniques, for example, correlation studies of the reflected elastically scattered light and the reflected one- and multiphoton fluorescence light, will be investigated to achieve a successful non-invasive feedback about the formation of a sharp focus deep in the scattering medium. The theoretical results will be validated with measurements on optically and geometrically, partly microscopically, accurately characterized static and non-static phantoms and on animalic ex vivo tissue. In case of success, this research work promises a series of very important applications, especially, microscopic imaging of biological tissue much deeper than is possible today.