High throughput cell separation device

目前，细胞和细胞亚群的分离通常是通过人工净化的磁性系统进行的。这些细胞被贴上特异性抗体和磁性颗粒的标签，被困在磁场中，然后在高压下再次冲洗出来。这一过程与细胞的高应激潜能有关，对敏感细胞类型（如人类干细胞）尤其有毒。另一种选择是使用邻近核心设施的细胞分选器进行净化。然而，这种方法不是无菌的，并且有很高的污染风险。相比之下，该装置能够通过表达表面标记物来非常温和地净化复杂的细胞群。这种净化是在低压下进行的，没有压缩或改变电荷。分离后分选细胞的纯度通常达到≥95%。该过程是完全无菌的，因为它在一个完全封闭的系统中工作，所以细胞可以在不添加抗生素的情况下保存。此外，初始设置后的细胞群分离是完全自动化的，这大大节省了时间。

Currently, the separation of cells and cell subsets is usually performed by a magnetic system with manual purification. The cells are labelled with specific antibodies coupled with magnetic particles, trapped in a magnetic field and then rinsed out again at high pressure. This procedure is associated with a high stress potential for the cells and is particularly toxic for sensitive cell types such as human stem cells. An alternative is purification using a cell sorter in the adjacent Core Facility. However, this method is not sterile and involves a high risk of contamination. The device applied for, on in contrast, is capable of very gently purifying complex cell populations by means of the expression of surface markers. This purification is carried out under low pressure, without compression or alteration of the electrical charge. The purity of the sorted cells usually reaches ≥ 95% after separation The process is completely sterile, since it works in a completely closed system, so that cells can be kept without the addition of antibiotics. Furthermore, the separation of the cell populations after initial setup is fully automated, which results in a significant time saving.