Development of functional composite membrane and its application to the materials for dental regeneration

在含钙磷离子的电解液中，采用水热-电化学法在钛板、棒材和钛网表面成功地形成了针状磷灰石。沉积的针状磷灰石均匀且垂直于基片表面。沉积磷灰石的形貌和结晶度可以通过电解液的组成和温度、电流密度和基质等条件进行调节，利用电化学石英晶体微天平实时监测磷灰石的电化学沉积过程。电化学磷灰石的沉积可能受电极和电解液界面离子扩散的控制，电流负载可能会降低其活化能。我们应用于牙科组织再生的…制备了一种新的由定向针状磷灰石和可生物降解聚合物组成的复合膜的制备方法更多的新药。复合膜的性能很容易控制；例如，这种膜的降解速度可以通过共聚物的厚度来调节，而厚度可以通过浓度和滴下体积来控制。结果表明，该复合膜与软组织和硬组织均具有良好的生物相容性。采用水热-电化学法将针状磷灰石包覆在钛网上，通过小鼠成骨样细胞MC3T3-E1的行为研究了其生物相容性。成骨细胞样细胞在沉积的磷灰石上附着和延伸，对细胞的发育无不良影响。在检测了碱性磷酸酶活性以及细胞外基质中的胶原和骨钙素后，电化学磷灰石涂层的钛网显示出比没有涂层的钛网更高的值。这些结果表明，在钛网上沉积的磷灰石具有良好的生物相容性。较少

Needle-like apatites were successfully formed on titanium plate, bar, and mesh by a hydrothermal-electrochemical method in an electrolyte containing calcium and phosphate ions. The deposited needle-like apatites were homogeneously and vertically oriented to the substrate surface. Morphology and crystallinity of the deposited apatite were easily regulated by the electrolytic conditions such as composition and temperature of electrolyte, current density, and substrate.The electrochemical deposition process of apatite was real-time monitored using an electrochemical quartz crystal microbalance. It seemed that the electrochemical apatite deposition may be controlled by ion diffusion at the boundary between the electrode and the electrolyte, and the electric current loading may reduce the activation energy for it.We applied to produce a novel fabrication method of a composite membrane consisting of oriented needle-like apatite and biodegradable polymer for tissue regeneration in dentistry a … More nd medicine. The properties of the composite membrane can be easily controlled ; e.g. the degradation rate of this membrane can be regulated by the thickness of the copolymer, which can be controlled by concentration and dropped volume. It was confirmed that this composite membrane has a good biocompatibility with soft tissue and hard tissue. Furthermore, BMP was applied on this composite membrane and produced the biologically active membrane.Needle-like apatites were coated on titanium mesh using hydrothermal-electrochemical method, and its biocompatibility was investigated through the behavior of mouse MC3T3-E1 osteoblast-like cells. The osteoblast-like cells attached and extended on the deposited apatite and no detrimental effects were observed on the development of the cell. After measuring alkaline phosphatase activity as well as collagen and osteocalcin of the extracellular matrix, the electrochemically apatite-coated titanium mesh indicated higher values than the titanium mesh without coating. These results suggest that the deposited apatite on titanium mesh has excellent biocompatibility. Less