Development of Myocardial Regenerative Therapy using Highly Regular Honeycomb-Patterned Scaffold inducing tissue self-regeneration

心肌梗死后心肌修复的细胞治疗是一种很有前途的新策略。然而，细胞疗法一般需要对细胞进行长时间的培养，以增加足够的细胞数量。为了解决这一问题，需要使用合适的支架来实现细胞的附着、增殖、分化等多种功能。在这项研究中，我们评估了细胞表面形貌对心肌细胞治疗行为的影响，使用高度规则的图案化生物降解膜通过简单的浇铸技术。与平面膜相比，蜂窝状图案膜显著促进了骨髓来源的间充质干细胞和成肌细胞的增殖。在拉伸的蜂窝状薄膜上，心肌细胞的形态正确地与拉伸方向对齐，而不是在正常的薄膜上对齐。在体内观察到蜂窝状薄膜的再细胞化，具有高度规整的孔洞大小和表面形貌的图案化薄膜对细胞的增殖、分化和排列具有很强的影响。蜂窝膜有望成为心肌再生治疗的一种新的支架材料

Cell therapy for myocardial repair after myocardial infarction is a new and promising strategy. However, cell therapy generally needs to culture the cell for a long period in order to increase a sufficient number of cells. To solve this problem, it needs to use a suitable scaffold for cell attachment, proliferation, differentiation and several functions. In this study, we evaluated the effect of surface topography in the cell for myocardial cell therapy behavior using the highly regular patterned biodegradable films by simple casting technique.The bon marrow-derived mesenchymal stem cell and myoblast proliferation was significantly accelerated on the honeycomb-patterned film as compared to the flat films. On the stretched honeycomb-patterned films, the cardiac cell morphology was correctly aligned to the stretch direction and not aligned on the normal films. Recellularization of honeycomb-patterned film was observed in vivo.The highly regular patterned films with controlled pore size and surface topography have a strong effect on the cell proliferation differentiation and alignment. The honeycomb-patterned films may be a novel candidate of the scaffold for Myocardial Regenerative Therapy