Genome-wide microsatellite mapping of multi-factorial ocular diseases.

为了确定眼病和高血压相关的易感基因，我们利用25000颗微卫星（microsatellite， MS）和汇总DNA进行了关联研究。每种疾病的进展情况如下。高度近视：534例。经过第一、二、三次筛选，156例阳性标记物通过。通过个体分型，获得26个阳性标记物。在每个候选区域周围进行SNP分型：收集了427份样本。完成1、2、3次MS筛选及个体基因分型，获得11个阳性标记物。SNP分型在每个候选区域周围进行。晶格变性：采集397个样品。在第一次筛选中，我们发现2851个标记显著相关。高血压：采集样本508份。完成MS筛选，获得135个阳性标记物。然后进行个体分型，通过55项指标。SNP基因分型正在进行。青光眼：192例。第一次筛查138例，阳性标记物占13.8%。现在正在执行第二个屏幕。同时，使用基因芯片进行SNP分型。

In order to identify susceptibility genes associated with ocular diseases and hypertension, we have performed association study, using 25000 microsatellites(MS) and pooled DNA. The progresses of each disease is as follows.High myopia : 534 samples were collected. After the first, second and third screen, 156 positive markers were passed. As a result of indivisual typing, 26 positive markers were obtained. SNP typings were performing in the surrounding each candidate regionBehcet disease : 427 samples were collected. The first, second and third MS screenings and individual genotyping were finished, and 11 positive markers were obtained. SNP typing are performing in the surrounding each candidate regions.Lattice degeneration : 397 samples were collected. In the first screening, we found significant association for 2851 markers.Hypertension : 508 samples were collected. MS screenings were finished and 135 positive markers were obtained. Individual typing then were performed, and 55 markers were passed. SNP genotyping are performing now.Glaucoma : 192 samples were collected. The first screen using 138 samples was performed and 13.8% positive markers were obtained. The second screen is performing now. Simultaneously, SNP typings using GeneChip are performing.