Genome-wide microsatellite mapping of multi-factorial ocular diseases.

多因素眼部疾病的全基因组微卫星图谱。

• 批准号: 14370562
• 负责人: MIZUKI Nobuhisa
• 金额: $ 7.68万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370562/
• 关键词: Genome; Microsatellite; SNP; Disease Genes; Mapping; Behcet disease; Cataract; Hypertension

The completion of the human genome project has led to worldwide interest in screening for genomic polymorphisms associated with disease susceptibility. For this to be accomplished, it is necessary to adopt efficient methods that probe the entire genome systematically, rapidly and accurately by polymorphic markers to identify specific genes with disease associated polymorphisms. Microsatellite (MS) is a repeated sequence consisting of generally two to six nucleotides (repeated unit), scattering throughout the human genome. MS characterized by a high degree of genetic polymorphisms in its number of repeated unit is expected to be an excellent marker in association analysis for evaluating disease susceptibility. The average number of alleles in MS is 10 (much more than that of SNP) and the average length of linkage disequilibrium of MS is 100kb (much longer than that of SNP). The best way to map the disease susceptibility gene, therefore, is first to narrow down candidate disease gene reg … More ions to 100 kb by genome-wide association mapping with MS, and then next to identify disease gene from these 100 kb candidate regions by conventional SNP analysis. We have collected and localized more than 30,000 MS markers in the human genome at 100 kb intervals and started pan genomic (genomewide) MS screening for identification of pathogenic genes of several diseases including hypertension, diabetes mellitus, open angle glaucoma (OAG), steroid glaucoma (steroid susceptibility gene), Behcet's diabetes mellitus, open angle glaucoma (OAG), steroid glaucoma (steroid susceptibility gene), Behcet's disease, high myopia, retinal lattice degeneration, age related macular degeneration (AMD), keratoconus, cataract, and sarcoidosis. Accordingly, we have so far found fifty to a hundred foci that may have hypertension susceptible genes. SNP analyses are under way to finally reach single disease genes. We are continuing MS screening, mapping and identification for the other diseases, demonstrating that our MS based strategy is quite efficient for genomewide mapping of diseases, especially complex or common diseases with multi-factorial basis. Less

人类基因组计划的完成引起了全世界对筛选与疾病易感性相关的基因组多态的兴趣。为此，有必要采用有效的方法，通过多态标记系统、快速、准确地探测整个基因组，以识别与疾病相关的特定基因的多态。微卫星(MS)是一个重复序列，通常由两到六个核苷酸(重复单位)组成，散布在人类基因组中。MS的重复单位数具有高度遗传多态的特点，有望成为关联分析中评价疾病易感性的优良标记。MS的平均等位基因数为10个(远多于SNP)，平均连锁不平衡长度为100kb(远大于SNP)。因此，绘制疾病易感基因图谱的最佳方法是首先缩小候选疾病基因reg…的范围。通过MS全基因组关联作图将更多的离子定位到100kb，然后通过常规的SNP分析从这100kb的候选区域中鉴定疾病基因。我们以100kb的间隔收集和定位了人类基因组中的30,000多个MS标记，并开始了泛基因组(基因组范围)的MS筛选，以确定几种疾病的致病基因，包括高血压、糖尿病、开角型青光眼(OAG)、类固醇青光眼(类固醇易感基因)、白塞氏糖尿病、开角型青光眼(OAG)、类固醇青光眼(类固醇易感基因)、白塞病、高度近视、视网膜网状变性、年龄相关性黄斑变性(AMD)、圆锥角膜、白内障和结节病。因此，到目前为止，我们已经发现了50到100个可能具有高血压易感基因的病灶。SNP分析正在进行中，以最终获得单一疾病基因。我们正在继续对其他疾病进行MS筛查、作图和鉴定，证明我们的基于MS的策略对于疾病，特别是具有多因素基础的复杂或常见疾病的全基因组作图是非常有效的。较少

项目成果

Cheng Q: "Cdk5/p35 and Rho-kinase mediate Ephrin-A5-induced signaling in retinal ganglion cells."Molecular and Cellular Neuroscience. 24(3). 632-645 (2003)

Cheng Q：“Cdk5/p35 和 Rho 激酶介导视网膜神经节细胞中 Ephrin-A5 诱导的信号传导。”分子和细胞神经科学。

Mizuki N: "Molecular genetics of Behcet Disease."Japan ophthalmologists association infoemation. 47. 58-66 (2003)

Mizuki N：“白塞病的分子遗传学。”日本眼科医师协会信息。

水木信久: "OTKとも"56 (2003)

水木信久：《OTK Tomo》56 (2003)

水木 信久: "ベーチェット病の病因と治療"OTKとも. 103. 18-24 (2003)

Nobuhisa Mizuki：“白塞氏病的病因学和治疗”OTK Tomo。103. 18-24 (2003)

野村 英一: "NEW MOOK眼科4,眼の自己免疫疾患"金原出版. 187 (2003)

野村荣一：“NEW MOOK 眼科 4，眼部自身免疫性疾病”Kanehara Publishing 187（2003）。