Measurement and control for multiscale-spatiotemporal neurodynamics

该项目对空间上三位数、时间上十位数的多尺度时空神经元动力学的测量和控制提出了挑战。该项目的目的是：在多电极阵列上长期培养，检测冲动，观察神经元网络的生长方式，并分析所获得的数据。因此，我们在多电极阵列上建立了长期的原代神经元分离细胞培养。此外，还建立了神经元主要部位蛋白质的免疫细胞化学染色。完成了构建实验系统攻克神经科学基本问题的主要课题。利用所构造的系统，我们得到了相关领域的重要问题。结果之一是导致同步爆发的存活神经元的最小密度。本工作的进一步发展，有望在未来的时空神经元动力学研究中取得丰硕的成果。

This project has challenged to measurement and control of multi-scale spatiotemporal neuronal dynamics, three-figure spatially and ten-figure temporally. The aim of the project is as follows ; long term culture on multi-electrode array, detection of impulses, observation of way of growing neuronal networks, and analysis of obtained data. As a result, we established long term primary neuronal dissociative cell culture on multi-electrode arrays. Moreover, immunocytochemistry of some proteins was also established for main parts of neuron during several month. The main subject of construction of experimental systems to attack basic problems in neuroscience was achieved. Using constructed system, we obtained important issues of the related field. One of the results is a minimum density of surviving neurons that caused synchronized bursts. Further development of the present works is expected to execute the future fruitful studies on spatiotemporal neuronal dynamics.