Multidisciplinary studies on the biological functions of GnRH peptidergic neurons

我们产生了GnRH 1，GnRH 2，和GnRH 3 GFP转基因青鳉可视化每个神经元在全脑体外制备。我们记录了自发电活动和对各种神经递质/神经调质物质的反应，并分析了它们的生理功能。我们还建立了一个转基因青鳉监测垂体促性腺激素的释放活动，并分析了激素释放的中枢机制。我们的研究成果使我们能够了解基于其进化历史的GnRH肽能神经元的多种生物学功能。

We generated GnRH1, GnRH2, and GnRH3 GFP-transgenic medaka to visualize each neuron in a whole brain in vitro preparation. We recorded spontaneous electrical activities and responses to various neurotransmitter/neuromodulator substances and analyzed their physiological functions. We also generated a transgenic medaka for monitoring the release activity of pituitary gonadotropic hormones and analyzed the central mechanisms of hormone release. Our research achievements enabled us to understand the multiple biological functions of GnRH peptidergic neurons that are based on their evolutionary history.