Multidisciplinary studies on the biological functions of peptidergic neuromodulatory systems : from molecules to behavior

肽能神经调节系统生物学功能的多学科研究:从分子到行为

基本信息

  • 批准号:
    15370032
  • 负责人:
  • 金额:
    $ 9.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

In the present research project, we obtained the following results concerning the biological functions of peptidergic neuromodulatory systems.1. Real-time simultaneous measurement of exocytotic release of GnRH peptide, electrical activities, and intracellular Ca^<2+> concentrationsAccording to our working hypothesis, changes in the sensory inputs from the environments are processed and fed on to the GnRH neuromodulatory system and modulate the release of GnRH peptides from the neurites of the terminal nerve GnRH neurons, which are distributed widely in the brain. Here, we developed a method for real-time simultaneous measurement of exocytotic release of GnRH peptide, electrical activities, and intracellular Ca^<2+> concentrations, using an in vitro brain block preparation with exposed GnRH neurons. We demonstrated a strong relationship between the pacemaker frequency and the intracellular Ca^<2+> concentrations, which are though to be related to the GnRH peptide release.2. Differential expression of GnRH receptor subtypes in the TN-GnRH neurons and the brainThe neuromodulatory functions of GnRH peptides released from the TN-GnRH neurons are thought to be mediated by GnRH receptors. Here, we cloned three subtypes of GnRH receptor genes from the brain of the dwarf gourami, and examined the distribution of GnRH receptor mRNAs by using the patch-clamp single-cell RT-PCR method, in which the cytoplasm of electrically recorded neurons are aspirated and analyzed by RT-PCR. We demonstrated differential expression of different GnRH receptor gene subtypes among different TN-GnRH neurons, which may suggest anto/paracrine regulation of GnRH neurons by GnRH peptides and the presence of subpopulation of TN-GnRH neurons. We also examined the anatomical distribution of GnRH receptor mRNAs expressions by using the in situ hybridization method.
在目前的研究项目中,我们获得了以下关于多肽神经调节系统的生物学功能的结果。实时同步测量GnRH肽的胞外释放、电活动和细胞内Ca^<2+>浓度根据我们的工作假设,来自环境的感觉输入的变化被处理并反馈给GnRH神经调节系统,并调节GnRH神经元的神经突释放GnRH肽,这些神经元广泛分布在大脑中。在这里,我们开发了一种实时同时测量GnRH肽的胞外释放、电活动和细胞内Ca^<2+>浓度的方法,使用暴露GnRH神经元的体外脑阻滞制剂。我们证明了起搏器频率与细胞内Ca^<2+>浓度之间存在很强的关系,而Ca^<2+>浓度与GnRH肽释放有关。GnRH受体亚型在n -GnRH神经元和大脑中的差异表达n -GnRH神经元释放的GnRH肽的神经调节功能被认为是由GnRH受体介导的。本研究从侏儒gourami的大脑中克隆了3个GnRH受体基因亚型,并采用膜片钳单细胞RT-PCR方法检测了GnRH受体mrna的分布,该方法通过抽吸电记录神经元的细胞质并进行RT-PCR分析。我们发现不同的GnRH受体基因亚型在不同的n -GnRH神经元中有差异表达,这可能表明GnRH肽对GnRH神经元的反/旁分泌调节以及n -GnRH神经元亚群的存在。我们还通过原位杂交方法检测了GnRH受体mrna表达的解剖分布。

项目成果

期刊论文数量(53)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Amano, M., Okubo, K., Yamanome, T., Oka, Y., Kawaguchi, N., Aida, K., Yamamori, K.: "Ontogenic development of three GnRH systems in the brain of a pleuronectiform fish, brain flounder."Zoological Science. (in press). (2004)
Amano, M.、Okubo, K.、Yamanome, T.、Oka, Y.、Kawaguchi, N.、Aida, K.、Yamamori, K.:“胸膜连接鱼大脑中三个 GnRH 系统的个体发育,
  • DOI:
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    0
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  • 通讯作者:
Ryouka Kawahara, Manabu Matsuda, Takao Mori: "Increase in the number of integrinbeta1-immunoreactive monocyte-lineage cells in experimentally-induced adenomyosis in mice."Life Sci. 73. 907-916 (2003)
Ryouka Kawahara、Manabu Matsuda、Takao Mori:“在实验诱导的小鼠子宫腺肌症中,整合素β1 免疫反应性单核细胞谱系细胞的数量增加。”生命科学。
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    0
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Ishikawa, M., Tsutsui, H., Cosson, J., Oka, Y., Morisawa, M.: "Strategies for sperm chemotaxis in the siphonophores and ascidians : a numerical simulation study."Biological Bulletin. (in press). (2004)
Ishikawa, M.、Ttsutsui, H.、Cosson, J.、Oka, Y.、Morisawa, M.:“管水母和海鞘中精子趋化性策略:数值模拟研究。”《生物通报》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Matsuda, M., Imaoka, T., Vomachka, AJ, Gudelsky, GA, Hou, Z., Mistry, M, Bailey, JP, Nieport, KM, Walther, DJ, Bader, M, Horseman, ND: "Serotonin regulates mammary gland development via an autocrine-paracrine loop."Dev Cell. 6. 193-203 (2003)
Matsuda, M.、Imaoka, T.、Vomachka, AJ、Gudelsky, GA、Hou, Z.、Mistry, M、Bailey, JP、Nieport, KM、Walther, DJ、Bader, M、Horseman, ND:“血清素调节
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
Strategies for sperm chemotaxis in the siphonophores and ascidians: A numerical simulation study
  • DOI:
    10.2307/1543540
  • 发表时间:
    2004-04-01
  • 期刊:
  • 影响因子:
    1.6
  • 作者:
    Ishikawa, M;Tsutsui, H;Morisawa, M
  • 通讯作者:
    Morisawa, M
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OKA Yoshitaka其他文献

OKA Yoshitaka的其他文献

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{{ truncateString('OKA Yoshitaka', 18)}}的其他基金

Experimental demonstration of functional compensation among paralogous genes encoding neuropeptides
编码神经肽的旁系同源基因功能补偿的实验证明
  • 批准号:
    24657050
  • 财政年份:
    2012
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Multidisciplinary studies on the biological functions of GnRH peptidergic neurons
GnRH肽能神经元生物学功能的多学科研究
  • 批准号:
    20247005
  • 财政年份:
    2008
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Comprehensive understanding of the multifunctional GnRH peptidergic nervous system
全面了解多功能GnRH肽能神经系统
  • 批准号:
    18370029
  • 财政年份:
    2006
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Physiological Analysis of Peptide Exocytosis and Pentidergic Neuromodulation.
肽胞吐作用和 Pentidergic 神经调节的分子生理学分析。
  • 批准号:
    12440237
  • 财政年份:
    2000
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development and Application of Selective Elcectochemical Analysys System for Peptides and Amines.
肽和胺选择性电化学分析系统的开发和应用。
  • 批准号:
    10554050
  • 财政年份:
    1998
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mechanisms of neural and endocrinological signal transduction systems during adaptation toenvironmental changes.
适应环境变化过程中神经和内分泌信号转导系统的机制。
  • 批准号:
    09640803
  • 财政年份:
    1997
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Physiological and Biochemical Studies on Neuromodulatory Action of Terminal Nerve GnRH System
终末神经GnRH系统神经调节作用的生理生化研究
  • 批准号:
    05804054
  • 财政年份:
    1993
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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基于溶酶体胞吐作用彻底阐明癌细胞对酸性环境的适应机制
  • 批准号:
    24K02225
  • 财政年份:
    2024
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Astrocytic exocytosis of ATP in amyloid pathology and Alzheimer's disease
淀粉样蛋白病理学和阿尔茨海默病中 ATP 的星形细胞胞吐作用
  • 批准号:
    10722422
  • 财政年份:
    2023
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    $ 9.41万
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Exocytosis of Plasmodium egress and invasion organelles
疟原虫出口和入侵细胞器的胞吐作用
  • 批准号:
    10888455
  • 财政年份:
    2023
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    $ 9.41万
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ArpC3-mediated actin remodeling in insulin granule exocytosis and diabetes
ArpC3 介导的肌动蛋白重塑在胰岛素颗粒胞吐作用和糖尿病中的作用
  • 批准号:
    10583734
  • 财政年份:
    2023
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    $ 9.41万
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Molecular Mechanisms Underlying Endothelial Weibel-Palade Body Biogenesis and Exocytosis
内皮 Weibel-Palade 体生物发生和胞吐作用的分子机制
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定义星形细胞溶酶体运输和胞吐作用在调节突触成熟中的作用
  • 批准号:
    10677414
  • 财政年份:
    2023
  • 资助金额:
    $ 9.41万
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Imaging exocytosis via a combined fluorescence and amperometry approach
通过荧光和电流分析相结合的方法对胞吐作用进行成像
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    2203359
  • 财政年份:
    2022
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    $ 9.41万
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    Standard Grant
Fusion pores in endocrine and synaptic exocytosis
内分泌和突触胞吐作用中的融合孔
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    10449673
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  • 资助金额:
    $ 9.41万
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The role of CAPS in the activity-dependent dense-core vesicle exocytosis: function and impairment
CAPS 在活性依赖性致密核心囊泡胞吐作用中的作用:功能和损伤
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  • 财政年份:
    2022
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    $ 9.41万
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Investigating the fundamental mechanisms of immune cell exocytosis
研究免疫细胞胞吐作用的基本机制
  • 批准号:
    RGPIN-2020-07139
  • 财政年份:
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  • 资助金额:
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  • 项目类别:
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