Elucidation of membrane dynamics and molecular mechanism of bone resorption in osteoclast by using multi-photon microscopy

骨是通过骨形成和骨吸收之间的平衡来维持的。破骨细胞是在人体骨吸收中起主要作用的细胞。为了阐明骨吸收的分子机制，本研究分析了破骨细胞的囊泡运输。通过进行4d成像，可以分析活破骨细胞中可能参与骨吸收的酸性囊泡的动态，不仅是水平的，而且是垂直的。

Bone is maintained by a balance between bone formation and resorption. The osteoclast is the cell that plays a major role in bone resorption in our body. In this study, to elucidate the molecular mechanism of bone resorption, vesicular trafficking in osteoclast was analyzed. By carrying out 4D-imaging, it becomes possible to analyze dynamics of acidic vesicles, probably involved in bone resorption, in living osteoclast not only horizontally but also vertically.