Development of the synthetic method of glycoproteins with homogenous glycan structure oriented toward functional analysis of glycans

在这项研究中，我们试图开发一种新的硫醇保护基团，用于保护Cys侧链。因此，Pocam基团可以设计、合成并用于固相肽合成和肽缩合反应。另一方面，我们尝试将细菌重组蛋白表达系统与体外糖基化反应相结合，合成新的糖蛋白。因此，我们可以制备含有特定官能团的重组蛋白。化学合成了一种携带s链聚糖的糖肽，这是最近发现的一种新的翻译后修饰。

In this study, we tried to develop a novel thiol protecting group which was useful for protecting Cys side chain. As a result, Pocam group could be designed, synthesized and used for solid-phase peptide synthesis and peptide condensation reactions. On the other hand, we tried to synthesize neo-glycoproteins by the combination of bacterial recombinant protein expression system and in vitro glycosylation reaction. As a result, we could prepare a recombinant protein containing a specific functional group. A glycopeptide carrying S-linked glycan which was recently found as a novel post-translational modification was chemically synthesized.