The regulation of cytoskeletal dynamics in endothelial cells during acute inflammation

在这个研究项目中，我研究了细胞因子诱导的激酶NUAK2在内皮细胞中的作用。当NUAK2在内皮细胞中被击倒时，肌动蛋白应激纤维丢失，肌球蛋白轻链磷酸化减少。此外，我在NUAK2中发现了几个与其激酶激活有关的自动磷酸化位点。我将针对NUAK2的反义吗啡注射到斑马鱼中，允许对血管进行实时成像，它们显示出致命的表型。这些数据表明，NUAK2-MYPT1轴参与了细胞骨架动力学的调节。

In this research project, I examined the role of cytokine-induced kinase NUAK2 in endothelial cells. When NUAK2 was knocked down in endothelial cells, loss of actin stress fiber and decrease in phosphorylation of myosin light chain were observed. Furthermore, I identified several auto-phosphorylation sites in NUAK2, which involved in activation of its kinase. I injected antisense morpholino against NUAK2 into the zebrafish allowing live imaging of blood vessels, and they showed lethal phenotypes. These data suggest that NUAK2-MYPT1 axis involves in the regulation of cytoskeletal dynamics in vitro and in vivo.