虚血性心筋を標的とするナノボディ修飾細胞外小胞を使用した心筋梗塞治療法の開発

我们已经生成了一个新的模块化合成纳米体噬菌体展示库，以选择包括CD8， PD-1和HER2在内的靶标。我们还通过液相色谱-质谱法鉴定了常氧和缺氧心肌细胞的差异蛋白。在这些差异蛋白中，CD29和CD166的差异最为显著，它们也参与了细胞在缺氧条件下的存活。CD29和CD166将被选择用于进一步的实验。

We have generated a novel modular synthetic nanobody phage display library to select for targets including CD8, PD-1 and HER2. We also identified differential proteins of normoxic and hypoxic cardiomyocytes by liquid chromatography mass spectrometry. Among these differential proteins, CD29 and CD166 were identified to have most significant difference and also be involved in cell survival in hypoxic conditions. CD29 and CD166 will be chosen for further experiments.