The roles of Nup88-protein interaction in metastasis and invasion of cancer cells.

目前还不清楚Nup88的过度表达是否会导致癌症的恶性进展。在这项研究中，我们筛选了Nup88结合蛋白，并研究了相互作用对癌症进展的影响。利用免疫沉淀技术，我们确定了波形蛋白，一个上皮-间充质转化的标志，作为Nup88的一个新的结合伙伴。波形蛋白在蛋白质水平的表达不受Nup88过表达的影响，反之亦然。Nup88与Vimentin的N-末端区域相互作用，这种相互作用保持Vimentin单体。由于单体Vimentin被报道为转录因子，我们推测Nup88的过表达通过调节Vimentin调控的基因转录而诱导肿瘤恶性转化。

It has been unclear whether Nup88 overexpression contributes to the malignant progression of cancer. In this study, we screened Nup88-binidng proteins and examined the effects of the interaction on cancer progression. Using an immunoprecipitation technique, we identified vimentin, a marker for epithelial-mesenchymal transformation, as a novel binding partner for Nup88. Vimentin expression at the protein level was unaffected by Nup88 overexpression and vice versa. Nup88 interacted with the N-terminal region of vimentin and the interaction kept vimentin monomeric. Since monomeric vimentin was reported to work as a transcriptional factor, we assume that Nup88 overexpression induces cancer malignancy via modulating the vimentin-regulated gene transcription.