Relationship between reproductive dysfunctions and oxidatively modified lipids in the circulation and follicular fluid of dairy cows

在泌乳奶牛中观察到的生殖功能障碍归因于代谢适应，这是确保泌乳能量需求所必需的。这在产后能量负平衡时期尤其重要，这段时间非常需要受孕。奶牛生殖功能障碍的潜在机制尚不完全清楚。6%至30%的奶牛会形成囊性卵泡（COF），这与不排卵有关，尤其是在产后早期。由于缺乏合适的模型来诱导和控制COF的形成，对COF的研究受到限制。在上一个资助期，我们开发了一个程序，以再现性诱导奶牛COF。我们还确定和表征了几个信号转导途径连接代谢应激，由于氧化应激与奶牛卵巢细胞的变化。这项资助提案的目的是诱导奶牛COF，并在分子和细胞水平上对所产生的改变进行生化、内分泌和细胞生物学表征。详细了解囊状卵泡的发生和发育可能提供一个起点，以制定战略，以挽救这种病理生理变化，从而提高奶牛的生殖健康。

Reproductive dysfunction observed in lactating cows has been attributed to metabolic adaptation which is needed to ensure energy demand for lactation. This is especially critical during the post-partum period of negative energy balance a time period where conception is highly desirable. The underlying mechanisms of reproductive dysfunction in dairy cows are incomplete understood. Between 6 and 30% of dairy cows develop a cystic ovarian follicle (COF) which is connected to anovulation, especially during the early post-partum period. Investigations on COFs are limited due to the lack of suitable models to induce and control COF formation. In the previous grant period we developed a procedure to reproducibility induce COF in dairy cows. We also identified and characterized several signal transduction pathways connecting metabolic stress due to oxidative stress with cellular alterations in the ovary of dairy cows. The aim of this grant proposal is to induce COF in dairy cows and to biochemically, endocrine and cell biologically characterize the resulting alterations on molecular as well as on cellular levels. A detailed knowledge of genesis and development of cystic ovarian follicles might offer a starting point to develop strategies in order to rescue this pathophysiological alteration and therefore improve reproductive fitness in dairy cows.