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Male fertility phenotypes: Characterization of two high fertility outbreed mouse lines to explore male factor (in)fertility.

雄性生育力表型：表征两个高生育力远交小鼠系，以探索雄性生育力因素。

基本信息

批准号：
234884451
负责人：
Privatdozent Dr. Joachim Weitzel
金额：
--
依托单位：
Institut für Fortpflanzungsbiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/234884451?language=en
关键词：
Male fertility phenotypes Characterization two

项目摘要

Infertility is a major medical problem worldwide. Most of the studies conducted to elucidate reasons for male factor infertility described single gene alterations. However, this does not reflect reality in most infertile patients. Infertility rather appears to be a complex, multi-causal phenomenon. In the present study we therefore change the perspective by using two worldwide unique mouse models (fertility lines FL1 and FL2) which were selected for the parameter "high fertility" for more than 150 generations. These mice almost doubled the number of offspring as well as they doubled the total birth weight per litter in comparison to a control line. The aim of this project is to initially characterize the testicular phenotypes of these two fertility lines. The characterization includes evaluation of the histological composition of the testis, expression of marker genes, hormone levels and classical sperm motility parameters. A global gene expression analysis will elucidate the molecular pathways affected by the phenotypic characteristics.We expect to gain new basic insights into molecular mechanisms within the testicular parenchyma which have a direct impact on the reproductive outcome.We hypothesise that those genes and pathways which are affected in order to maintain a higher fertility also contribute to the phenotype "reduced fertility" upon their alteration.Therefore, with this project we build the basis for future investigations concerning pathogenesis and therapy of male factor infertility.

不孕不育是世界范围内的一个重大医学问题。为了阐明男性因素不育症的原因，大多数研究都描述了单基因改变。然而，这并不能反映大多数不孕症患者的实际情况。不孕不育似乎是一种复杂的、多种原因的现象。因此，在本研究中，我们通过使用两个世界上独一无二的小鼠模型(繁殖系FL1和FL2)来改变这一观点，这两个模型是在超过150代的时间里被选为高繁殖力参数的。与对照品系相比，这些小鼠的后代数量几乎翻了一番，每窝的总出生体重也翻了一番。本项目的目的是初步确定这两个可育系的睾丸表型。其特征包括评估睾丸的组织结构、标记基因的表达、激素水平和经典的精子运动参数。全球基因表达分析将阐明受表型特征影响的分子途径。我们希望对睾丸实质内直接影响生殖结果的分子机制获得新的基本见解。我们假设，那些为了保持较高生育率而受到影响的基因和途径也会在它们发生变化时导致表型生育能力下降。因此，通过这个项目，我们为未来研究男性因素不育症的发病机制和治疗奠定了基础。