Tumor oxygenation to in crease sensitivity to anti-cancerous drug by using artificial oxygen carrier

癌细胞通常对抗癌剂具有抗性。为研究人工氧载体（AOCs）是否能增加肿瘤细胞对抗癌药物的敏感性，将Yoshida肉瘤细胞（LY-80）移植于大鼠背部肌肉，用微球法对肿瘤内血流进行定量分析，用Oxyspot法测定肿瘤内的血流量，并与对照组比较。测定肿瘤组织氧分压（PtO_2）。使用的AOC是血红蛋白囊泡（HbV）、白蛋白-血红素（AH）和全氟化碳（PFC）。他们被静脉注射。作为对照，注射Ractate Ringer溶液和5%白蛋白溶液。AOCs中只有AH使PtO_2增加到基础值的130%。另一组PtO_2无明显增加，可能是清除NO引起微血管收缩，使血流量减少，其机制有待进一步实验研究。还必须评估AOC和抗癌药物的动脉输注。

Cancer cells are often resistant to anti-cancerous agents. One of the reasons of this phenomenon is cancer cells are hypoxic.To study if artificial oxygen carriers (AOCs) increases sensitivity of tumor cells to anti-cancerous agents, following experiments were designed.Yoshida sarcoma cells (LY-80) were inplanted to the back muscle of rats.Quantative analysis of blood flow in the tumors was performed by microsphere method.Using Oxyspot method, tissue oxygen pressure of the tumors (PtO_2) was evaluated. The AOCs used were hemoglobin vesicles (HbV), albumin-heme (AH) and perfluorocarbons (PFCs). They were intravenously injected. As control, Ractate Ringer solution and 5% albumin solution were injected. Among AOCs, only AH increased PtO_2 upto 130% of the basal value. In another groups PtO_2 was not increased, probably become they scavenged nitric oxide to cause microvascular contraction to decrease blood flow.To resolve this mechanism further experiments are needed to study microvascular circulation in the tumors. Also arterial infusion of the AOCs and anti-cancerous drugs must be evaluated.