T-cell targeting treatment strategy of infective or allergic inflammation in nasopharyrix-an analysis of human T -lymphocytes recruting to nasopharyngeal mucosal linings-

我们利用分子生物学技术和实验动物，如tcr转基因小鼠、IL-5转基因小鼠、NOD-scid小鼠，从免疫学角度研究了人类和啮齿类动物鼻咽部粘膜免疫诱导和维持的精确机制。在动物实验中，我们已经阐明了募集到鼻咽粘膜的T细胞和B细胞对于诱导和维持抗原特异性粘膜抗体反应是必不可少的，并且这些细胞因子谱的T细胞修饰可能控制鼻咽粘膜过敏性或感染性炎症的情况。另一方面，我们对人类样本进行的定量RT-PCR分析显示，鼻过敏患者和感染性鼻炎患者鼻黏膜T细胞细胞因子谱存在某种差异，并且对NOD-scid小鼠进行的人类扁桃体淋巴细胞转移研究表明，根据持续的抗原刺激，人类扁桃体淋巴细胞已被承诺为产生抗体的B细胞和记忆T细胞。这些结果可能鼓励我们进行广泛的研究，以寻求治疗鼻咽部炎症性疾病的新策略和临床应用。

We have immunologically examined the precise mechanism of induction and maintenance of mucosal immunity in nasopharynx of human-beings and rodents as well, by employing molecular biology techniques and experimental animals such as TCR-transgenic mice, IL-5 transgenic mice, and NOD-scid mice. In animal experiments, we have elucidated that T cells recruting to the nasopharyngeal mucosa as well as B cells are essential for an induction and maintenance of antigen-specific mucosal antibody response and that these T-cell modification in view of cytokine profile may control the scenario of allergic or infective inflammation in nasopharyngeal mucosa. On the other hand, our quantitative RT-PCR analysis with human samples revealed that T-cell cytokine profile in nasal mucosae is different somehow between patients with nasal allergy and those with infective rhinitis and a transfer study of human tonsillar lymphocytes to NOD-scid mice demonstrated that human tonsillar lymphocytes have been committed to be antibody-prodecing B cells and memory T cells according to the continuous antigenic stimuli. These results taken together might encourage our extensive research for pursuiting the new strategy and clinical application of it to the treatment of inflammatory diseases in nasopharynx.