Development of a new measure for controlling pine wilt disease using oviposition deterrents of insect vector, Monochamus alternatus

墨天牛交替雌虫在交配后立即在卵附近沉积存款类囊状物质。这种物质使同种雌性不敢反抗。雌虫存活时长形受精囊腺内有无色至淡黄色透明液体，雌虫死亡后变为琥珀色透明弹性物质，与对侧瘢痕内的类琥珀色物质极为相似。受精囊腺的甲醇提取物阻止了雌性的反对。切片显示受精囊腺内的液体位于交配囊内。结果表明，受精囊腺产生产卵抑制物质。受精囊腺甲醇提取物经丙酮和甲醇分级后，甲醇不溶物对产卵有抑制作用。浓缩后用冷甲醇处理，将活性组分分为甲醇不溶性组分（包括蛋白质）和甲醇溶性组分。两个组分均不产卵- ...更多信息 威慑作用它们的混合物不能恢复活性。新鲜幼虫粪便的甲醇提取物阻止女性反对。萃取物用苯、氯仿、丙酮和甲醇分级。由于只有苯可溶性组分表现出产卵忌避作用，通过连续的硅胶柱色谱法将其分成6个组分。其中己烷可溶性组分具有产卵忌避作用。用柱层析法将己烷溶性组分分成5个组分。由于得到的苯可溶性和甲醇可溶性级分显示出产卵抑制作用，因此对这两种级分进行TLC分析，其中分别观察到7个和3个斑点。苯馏分中的一个斑点的物质表现出产卵忌避作用。GC和GC-MS显示存在6种化合物。因此确定苯乙酮表现出最大的产卵忌避效果的化合物，但没有统计学意义。少

Monochamus alternates females deposit jelly-like substance near the eggs just after opposition. The substance deterred the conspecific females from opposition. Colorless to pale yellow, transparent liquid was present in the long spermathecal gland of living females and changed to amber, transparent, elastic substance after females died, which greatly resembled jelly-like substance in opposition scars. Methanol extract of spermathecal gland deterred females from opposition. Section showed that the liquid in spermathecal gland was found in bursa copulatrix. The facts showed that spermathecal gland produced the oviposition deterrents. When methanol extract of spermathecal gland was fractionated with acetone and methanol, methanol-insoluble fraction showed an oviposition-deterring effect. Treatment with cold methanol after concentration divided the active fraction into methanol-insoluble fraction including protein and methanol-soluble fraction. Neither of the two fractions had oviposition- … More deterring effect. A mixture of them could not recover the activity. Methanol extract of fresh larval frass deterred females from opposition. The extract was fractionated with benzene, chloroform, acetone, and methanol. As only the benzene-soluble fraction showed an oviposition-deterring effect, it was fractionated into six fractions by successive silica gel column chromatography. Hexane-soluble fraction of them had an oviposition-deterring effect. The hexanesoluble fraction was partitioned into five fractions by column chromatography. As the resulting benzene soluble and methanol-soluble fractions showed an oviposition-deterring effect, both fractions were subjected to TLC analysis, where 7 and 3 spots were observed, respectively. Substances of one spot from benzene fraction showed an oviposition-deterring effect. GC and GC-MS revealed the presence of 6 compounds. Acetophenone thus identified showed the greatest oviposition-deterring effect among the compounds although there was no statistical significance. Less