The Studies towards Inhibition of Joint Adhesion by Decorin Gene Transtection Techrique

最近，人们已经知道，生长因子不仅在组织的愈合过程中有很大的作用，而且在活体内几种疾病的进展中也有很大的作用。在骨科领域，已有研究表明，转化生长因子-β等生长因子在肌腱粘连、肩关节僵硬疼痛、股骨头痉挛等疾病中起核心作用。首先，我们进行了以下实验，以了解抑制转化生长因子-β活性是否可以防止创面愈合过程中粘连的形成。(1)用渗透压泵将转化生长因子-β的单抗注入兔膝关节内粘连模型，石膏固定4周后，用X-P法评价膝关节屈曲痉挛状态，并对粘连部位进行大体和组织学评价。结果显示，抗转化生长因子-β抗体注射组的肌挛缩角明显低于假手术组和载体载体注射组。另一方面，I…更多的研究表明，核心蛋白聚糖是蛋白多糖家族的成员之一，可以结合并降低转化生长因子-β活性。因此，预计在伤口愈合过程中注射核心蛋白聚糖可以减少粘连的形成。为了解核心蛋白聚糖在创面愈合模型中的作用，进行了以下实验。(研究2)从牛关节软骨中分离出3种浓度的核心蛋白聚糖(10，50,250μg/mlPBS)，按研究1的方法连续注射4周。结果显示，注射核心蛋白聚糖的三组动物的收缩角度均明显低于未注射对照组，且高浓度组的收缩角度明显小于未注射的对照组。因此，我们认为持续注射抗转化生长因子-β抗体和核心蛋白聚糖可以减少创面愈合过程中的粘连形成。我们最初的目标是利用核心蛋白聚糖基因导入技术防止粘连的研究，现在正在进行中，应该在不久的将来就能实现。较少

Recently, it has been known that growth factors involved greatly not only in the healing process of the tissue but also in the progression of several morbid conditions in a living body. Of the orthopaedic area, it has been indicated that growth factors like as TGF-β played central roles in the condition of tendon adhesion, stiff and painful shoulder, Dyupytren contructure. At first, we studied following experiment to know whether suppression of TGF-β activity can prevent adhesion formation in the wound healing. (Study 1) Monoclonal antibody of TGF-β was injected to the intra-articular adhesion model of the rabbit knee using osmotic pumps and the joint was gypsed for 4 weeks, then flexion contracture evaluation by X-P, macroscopic and histological evaluation of the adhesion site were done. The results showed that the contracture angles of anti-TGF-β antibody injected group were significantly lower than those of sham operation group or carrier vehicle injected group. On the other hand, i … More t is known that decorin, one of the members of proteoglycan family, binds and decreases TGF-β activity. Therefore, it is expected that adhesion formation can be reduced by decorin injection in the wound healing process. To know the effects of decorin in the wound healing model, following experiment was done. (Study 2) Three concentrations of decorin (10,50,250 μg/ml PBS) isolated from bovine articular cartilage were injected continuously and gypsed for 4 weeks in the same manner as study 1. The results showed that all three groups injected decorin had significantly lower contracture angles than non-injected control group, and its angles were much smaller in higher concentration group. Therefore, we concluded that continuous injection of anti-TGF-β antibody and decorin can reduce the adhesion formation in the process of wound healing. The studies concerning to adhesion prevention using decorin gene transfection technique, which at first we aimed, were now on going and should be realized in the near future. Less