Study of the occurrence mechanism of adhesion tissues in the temporomandibular joint

为了研究颞下颌关节内错位(ID)的粘连，采用凝胶收缩模型，观察了正常关节盘(D)、滑膜(S)和两种类型的ID粘连组织(带状粘连[BA]和假墙状粘连[PW])中成纤维细胞样细胞在TMJ中的粘连能力。连续12天的凝胶直径测量表明，6种细胞连续收缩CG。在TMJ相关细胞中，PW的收缩能力最强。收缩能力的差异可能反映了它们的增殖潜力、迁移活性、细胞因子的产生和反应以及细胞外基质产物的合成。收缩强度大小顺序为：NS&GT；PW&GT；BA&GT；D/GF&GT；细胞排列：MTD ECM组分在两种粘连中用透射电子显微镜、扫描电子显微镜和罗丹明标记的苯并胺染色观察。基底动脉内可见少量成纤维细胞样细胞，具有粗大的丝状突起和…区。胶原束排列更整齐。胶原束与粘连延伸的方向平行。PW内可见大量活跃的成纤维细胞样细胞，有许多细小的丝状足突，主要为排列不规则的胶原束。细胞外基质内可见部分假壁样粘连、钙化的胶原纤维。在关节镜活检中表现出最高抵抗力的粘连由钙化胶原纤维组成。此外，还研究了日本广泛使用的抗过敏药物N-(3，4-二甲氧基肉桂酰基)邻氨基苯甲酸对凝胶收缩过程的影响。曲尼司特对两种类型的成纤维细胞样细胞的抑制程度不同；BA对曲尼司特的反应比对其反应强得多。曲尼司特的反应程度不同的原因尚不清楚，尽管这可能反映了曲尼司特受体表达水平的差异或每种细胞类型对细胞因子的反应。较少

To characterize the adhesions in internal derangement (ID) of the temporomandibular joint (TMJ), we used a gel contraction model to investigate the ability of fibroblast-like cells derived from human normal articular disc (D), synovium (S) and two types of adhesion tissue of ID in the TMJ (band-like adhesion [BA], pseudowall-like adhesion [PW]).Sequential measurements of gel diameter for 12 days showed that six kinds of cells contracted CG continuously. Among TMJ-related cells, PW had the greatest contraction potency. Differences in ability of the contraction potency may reflect their proliferative potential, migratory activity, production of and response to cytokines, and synthesis of extracellular matrix products. The order of contraction potency is ; NS>PW>BA>D/GF>SArrangement of cells mtd ECM components were examined in two types of adhesions by TEM, SEM and rhodamine-labeled phalloidine staining. In BA, there were a few fibroblast-like cells with thick filopodia processes and orde … More rly arranged collagen bundles were prominent. The collagen bundles ran parallel to direction in which the adhesion was extended. In PW, there were a lot of active fibroblast-like cells with many fine filopodia processes and randomly arranged collagen bundles were mainly seen. A part of pseudowall-like adhesion, calcified collagen fibrils were observed in the extracellular matrix. The adhesions that showed the highest resistance during arthroscopic biopsy consisted of calcifiecl collagen fibrils. This structure in internal derangement may strongly inhibit movement of the disc and joint.In addition the effects of tranilast, N-(3,4-dimethoxycinnamoyl) anthranilic acid, a widely used antiallergy drug in Japan on the gel contraction process was also investigated. The degree of inhibition induced by tranilast was not equal in the two types of fibroblast-like cell ; BA showing a much greater response to tranilast than PW.The reasons for the difference in magnitude of response to tranilast are unclear, although it may reflect differences in the level of the receptor expression for tranilast or response to cytokines by each cell type. Less