Identification of the susceptive genes for atopic diseases using positional candidate gene approaches.

儿童期发作的哮喘常与特应性反应有关。我们使用398个标记对47个日本家庭（197个成员）进行了全基因组连锁搜索，这些家庭有两个以上的螨虫敏感的特应性哮喘患者（65对受影响的兄弟姐妹）。使用MAPMAKER/SIB程序对特应性哮喘作为数量性状进行多点连锁分析。我们观察到5q31-q33上最大负荷评分（MLS）为4.8的显著关联证据。此外，还获得了MLS=2.7的4q35和MLS=2.4的13q11的暗示性证据。其他可能的连锁区域包括6p22-p21.3 （MLS=2.1）、12q21-q23 （MLS=1.9）和13q14.1-q14.3 （MLS=2.0）。此外，我们观察到5q31-q33和12q21-q23与特应性皮炎相关的提示证据。这些数据提示5q31-q33和12q21-q23染色体区域可能是特应性哮喘和特应性皮炎的常见易感区域。

Childhood-onset asthma is frequently found in association with atopy. We conducted a genomewide linkage search in 47 Japanese families (197 members) with more than two mite-sensitive atopic asthmatics (65 affected sib-pairs) using 398 markers. Multipoint linkage analysis was carried out for atopic asthma as a quantitative trait using the MAPMAKER/SIB program. We observed significant evidence for linkage with maximum lod scores (MLS) of 4.8 on 5q31-q33. In addition, suggestive evidence on 4q35 with MLS=2.7 and on 13q11 with MLS=2.4 was obtained. The other possible linkage regions included 6p22-p21.3 (MLS=2.1), 12q21-q23 (MLS=1.9), and 13q14.1-q14.3 (MLS=2.0). Furthermore, we observed suggestive evidence for linkage with atopic dermatitis on 5q31-q33 and 12q21-q23. These data suggest that the chromosome region of 5q31-q33 and 12q21-q23 may be common susceptive region of atopic asthma and atopic dermatitis.