Regulation of cell division by ubiquitin-dependent proteolysis and by spindle assembly checkpoint

分析了后期促进复合体(APC)的调控机制。研究发现，APC的活性不仅受其调节因子CDc20和CDH1与APC的结合，而且还受APC自身和CDc20和CDH1的磷酸化的调节。APC10/doc1被鉴定为APC亚基之一。SLK被认为是PLK的上游激酶，调节有丝分裂的不同阶段。鉴定了一种新的动粒蛋白CENP-H。CENP-H在整个细胞周期中仅在活跃的动粒上定位于着丝粒内板，并形成多聚体并与MCAK结合，被认为调节着染色体的分离。CENP-H被发现是基本动粒结构所必需的，因为CENP-H的缺失导致了动粒的关键基本成分之一CENP-C的消除。

Regulatory mechanism of anaphase promoting complex (APC) was analyzed. It was found that APC activity was regulated not only by binding of its regulatory factors Cdc20 and Cdh1 to APC, but also by phosphorylation of APC itself and Cdc20 and Cdh1. APC10/Doc1 was identified as one of the APC subunits. Slk was identified as an upstream kinase of plk, which regulates various stages of mitosis. A novel kinetochore protein CENP-H was identified. The CENP-H was specifically localized in inner kinetochore plate only at active kinetochore throughout the cell cycle, and formed multimer by itself and bound MCAK which was thought to regulate choromosome separation. CENP-H was found to be essential for basic kinetochore structure, since delection of CENP-H resulted in elimination of CENP-C, one of the key basic constituents in kinetochore.